Regulation of spindle orientation and neural stem cell fate in the Drosophila optic lobe

doi:10.1186/1749-8104-2-1

Neural Development

Volume 2

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Research article

Regulation of spindle orientation and neural stem cell fate in the Drosophila optic lobe

Boris Egger¹^* , Jason Q Boone²^* , Naomi R Stevens¹ , Andrea H Brand¹ and Chris Q Doe²

¹The Wellcome Trust/Cancer Research UK Gurdon Institute and Department of Physiology, Development and Neuroscience, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK

²Institute of Molecular Biology, Institute of Neuroscience, Howard Hughes Medical Institute, University of Oregon, Eugene, OR 97403, USA

author email corresponding author email* Contributed equally

Neural Development 2007, 2:1doi:10.1186/1749-8104-2-1


Published:	5 January 2007

Additional files

Additional data file 1:

scute and mira mRNA expression in the optic lobe. (a) FISH to detect sc mRNA (green) in late third instar in combination with immuno-staining for Dlg (red). sc mRNA is expressed in the entire epithelium and in the medial neuroblasts. The 3.7sc-lacZ reporter line (Figure 2b) is expressed strongly in the neuroepithelium and is downregulated in medial neuroblasts. 3.7sc-lacZ may not reproduce the entire sc expression pattern, or lacZ expression in medial neuroblasts may be below our detection level. (b) FISH for mira mRNA (green) in late third instar in combination with immuno-staining for Dlg (red). mira mRNA is expressed in medial neuroblasts but not in neuroepithelial cells.

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Additional data file 2:

Optic lobe neuroblasts divide asymmetrically. Asymmetric division of a medial OPC neuroblast in a late third instar brain. GAL4^c855adrives Pon-GFP and H2B-mRFP1 in the OPC progenitor cells.

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Additional data file 3:

Unequal segregation of Pon-GFP to the GMC daughter cell. Asymmetric division of an OPC neuroblast in a late third instar brain. GAL4^c855adrives Pon-GFP and H2B-mRFP1 in the OPC progenitor cells. Note that Pon-GFP is asymmetrically segregated to the basal daughter cell

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Additional data file 4:

Optic lobe neuroepithelial cells divide symmetrically. Symmetric division of an OPC neuroepithelial cell in late third instar. GAL4^c855adrives Pon-GFP and H2B-mRFP1 in OPC progenitor cells. Note that Pon-GFP is symmetrically segregated to both daughter cells

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