Additional data File 3.
Equivalent numbers of bipolar cells, Müller glia, horizontal cells and cone photoreceptors develop in wild-type and Zac1 mutant retinal explants, while the number of amacrine cells increased in Zac1 mutant retinae. E18.5 wild-type (a,c,e,g,i,k,m,o,q) and Zac1-deficient (b,d,f,h,j,l,n,p,r) retinae were cultured 8DIV and labelled with Chx10 (red, a,b) for bipolar cells, p27Kip1 (red, c,d) and CRALBP (red, e,f) for Müller glia, peanut agglutinin (PNA, green, g,h) and s-opsin (i,j) for cones, Bhlhb5 (green, k,l) for GABAergic amacrine cells, calbindin for horizontal cell bodies (red, with processes in outer plexiform layer; m,n) and AII amacrine cells (deeper in INL; m,n), and GABA (red, o,p) and GlyT1 (red, q,r) for amacrine cell subtypes. Explants were counterstained with DAPI (blue).
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Ma et al. Neural Development 2007 2:11 doi:10.1186/1749-8104-2-11