Additional data File 4.

RGC differentiation is unperturbed in Zac1-deficient retinae at E18.5. Brn3a (a,b) and Brn3b (c,d) immunolabeling of RGCs in wild-type (a,c) and Zac1 mutant (b,d) retinae at E18.5. Quantitation of Brn3a (e) and Brn3b (f) expressing cells revealed equivalent numbers of RGCs in wild-type (n = 3 retinae; black bar) and Zac1 mutant (n = 3 retinae; white bar) retinae. Brn3a (p = 0.95; wild-type: 6.4 ± 1.0% retinal cells; 774 Brn3a+/12138 DAPI+; Zac1 mutant: 7.4 ± 0.4%; 743 Brn3a+/10123 DAPI+) and Brn3b (p = 0.23; wild-type: 3.3 ± 0.7%; 269 Brn3b+/6813 DAPI+; Zac1 mutant: 5.0 ± 0.7%; 393 Brn3b+/7818 DAPI+).

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Ma et al. Neural Development 2007 2:11   doi:10.1186/1749-8104-2-11