Additional data File 5.
Amacrine cell precursors do not undergo more apoptosis or divide ectopically in Zac1 mutant retinae. (a-d) E10.5 (a,b) and E15.5 (c,d) retinae immunostained for activated caspase-3 (ac-3) (red) in wild-type (a,c) and Zac1+m/-(b,d) embryos. Inserts in c,d are high magnification images of ac-3+ cells. (e-h) Ac-3 (red)/Pax6 (green) double+ cells label apoptotic amacrine cells in E18.5→4DIV explants. g and h are high magnification images of boxed areas in e and f, respectively. Ac-3+ amacrine cells are marked by arrowheads (g,h). (i) Percentage of Pax6+/ac-3+ apoptotic amacrine cells in wild-type (black bars; 45 ac-3/Pax6 double+/2925 Pax6+) and Zac1+m/- (white bars; 22 ac-3/Pax6 double+/2538 Pax6+) E18.5→4DIV explants. (j) Percentage of BrdU+/Pax6+ dividing amacrine cells in total Pax6+ population in E18.5 explants cultured 1DIV, 2DIV and 4DIV. 1DIV (p = 0.40; wild-type: 1.1 ± 0.3%; n = 3 explants; 12 BrdU/Pax6 double+/1071 Pax6+; Zac1 mutant: 0.7 ± 0.2%; n = 3 explants; 10 BrdU/Pax6 double+/1386 Pax6+), 2DIV (p = 0.76; wild-type: 0.9 ± 0.3%; n = 3 explants; 16 BrdU/Pax6 double+/1698 Pax6+; Zac1 mutant: 0.8 ± 0.3%; n = 3 explants; 16 BrdU/Pax6 double+/1983 Pax6+) and 4DIV (p = 0.44; wild-type: 0.2 ± 0.2%; n = 3 explants; 5 BrdU/Pax6 double+/712 Pax6+; Zac1 mutant: 0.4 ± 0.3%; n = 3 explants; 16 BrdU/Pax6 double+/2307 Pax6 single+). Blue is DAPI counterstain.
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Ma et al. Neural Development 2007 2:11 doi:10.1186/1749-8104-2-11