Additional data File 8.
Amacrine cell marker expression domains are expanded in E18.5 Zac1 mutant retinal explants cultured 4 DIV. Marker expression in E18.5 retinal explants cultured 4 DIV from wild-type (a,c,e,g,i,k,m) and Zac1-deficient (b,d,f,h,j,l,n) embryos. Chx10 transcript (a,b) and Chx10 protein (c,d) distribution in retinal explants. Crx (e,f), Hes1 (g,h),Six3 (i,j), Barhl2 (k,l) and Math3(m,n) expression. Explants processed for Hes1 and Six3 RNA in situ hybridization were also immunolabeled with anti-BrdU (after 30 min exposure) to label dividing cells.
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Ma et al. Neural Development 2007 2:11 doi:10.1186/1749-8104-2-11