Research article
Lola regulates Drosophila olfactory projection neuron identity and targeting specificity
1 Howard Hughes Medical Institute, Department of Biological Sciences, Stanford University, Stanford, California 94305, USA
2 Howard Hughes Medical Institute, Department of Bioengineering, Stanford University, Stanford, California 94305, USA
3 Department of Biomedical Engineering, Emory University, Atlanta, Georgia 30322, USA
4 Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA
5 National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA
Neural Development 2007, 2:14 doi:10.1186/1749-8104-2-14
Published: 16 July 2007Additional files
Additional file 1:
Additional lola mutant allele MARCM analysis demonstrating dendritic targeting defects. Supplemental Figure S1 showing targeting defects in lolaore5D2, lolaorc4, and lolaore119 mutant PNs.
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Additional file 2:
Supplemental Tables S1-S6. Supplemental tables and legends referenced in the main text. This includes a semi-quantitative analysis of axon targeting defects in lola-/- and lolaORE5D20-/-, a complete summary of DL1 dendrite and axon phenotypes by allele as percentage affected, a complete summary of vPN dendritic phenotypes by allele as percentage targeted correctly, clonal frequencies for MARCM experiments and primer sequences for in situ hybridization probes.
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Additional file 3:
Additional lola isoform in situ in the Drosophila brain. Supplemental Figure S2 showing controls and verification for our in situ technique and additional lola isoform hybridization results.
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Additional file 4:
Additional lola isoform RT-PCR analysis in the Drosophila brain. Supplemental Figure S3 showing addition LCM RT-PCR experiments comparing lola isoform expression at different developmental time points and in different brain tissues.
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Additional file 5:
Effects of UAS-lola overexpression on adPN and lPN dendrites and axons. Supplemental Figure S4 showing adPN and lPN phenotypes in MARCM clones in a wild-type background expressing UAS-lola A, UAS-lola L and UAS-lola T.
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Additional file 6:
adNB and lNB phenotypes in lola-/-, UAS-lola MARCM clones. Supplemental Figure S5 showing adPN and lPN phenotypes in lola-/- clones expressing UAS-lola A, UAS-lola L and UAS-lola T.
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Additional file 7:
Protocol for RNA in situ hybridization in fly larval/pupal/adult tissues. Our detailed protocol for performing RNA in situ hybridization on Drosophila at later developmental time points.
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Additional file 8:
Legends for supplemental Figures S1-S5. All supplemental figure titles and legends.
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