Lola regulates Drosophila olfactory projection neuron identity and targeting specificity1Howard Hughes Medical Institute, Department of Biological Sciences, Stanford University, Stanford, California 94305, USA 2Howard Hughes Medical Institute, Department of Bioengineering, Stanford University, Stanford, California 94305, USA 3Department of Biomedical Engineering, Emory University, Atlanta, Georgia 30322, USA 4Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA 5National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA
Neural Development 2007, 2:14doi:10.1186/1749-8104-2-14
Additional filesAdditional file 1: Additional lola mutant allele MARCM analysis demonstrating dendritic targeting defects. Supplemental Figure S1 showing targeting defects in lolaore5D2, lolaorc4, and lolaore119 mutant PNs. Format: PDF Size: 178KB Download file This file can be viewed with: Adobe Acrobat Reader Additional file 2: Supplemental Tables S1-S6. Supplemental tables and legends referenced in the main text. This includes a semi-quantitative analysis of axon targeting defects in lola-/- and lolaORE5D20-/-, a complete summary of DL1 dendrite and axon phenotypes by allele as percentage affected, a complete summary of vPN dendritic phenotypes by allele as percentage targeted correctly, clonal frequencies for MARCM experiments and primer sequences for in situ hybridization probes. Format: DOC Size: 186KB Download file This file can be viewed with: Microsoft Word Viewer Additional file 3: Additional lola isoform in situ in the Drosophila brain. Supplemental Figure S2 showing controls and verification for our in situ technique and additional lola isoform hybridization results. Format: PDF Size: 301KB Download file This file can be viewed with: Adobe Acrobat Reader Additional file 4: Additional lola isoform RT-PCR analysis in the Drosophila brain. Supplemental Figure S3 showing addition LCM RT-PCR experiments comparing lola isoform expression at different developmental time points and in different brain tissues. Format: PDF Size: 80KB Download file This file can be viewed with: Adobe Acrobat Reader Additional file 5: Effects of UAS-lola overexpression on adPN and lPN dendrites and axons. Supplemental Figure S4 showing adPN and lPN phenotypes in MARCM clones in a wild-type background expressing UAS-lola A, UAS-lola L and UAS-lola T. Format: PDF Size: 127KB Download file This file can be viewed with: Adobe Acrobat Reader Additional file 6: adNB and lNB phenotypes in lola-/-, UAS-lola MARCM clones. Supplemental Figure S5 showing adPN and lPN phenotypes in lola-/- clones expressing UAS-lola A, UAS-lola L and UAS-lola T. Format: PDF Size: 128KB Download file This file can be viewed with: Adobe Acrobat Reader Additional file 7: Protocol for RNA in situ hybridization in fly larval/pupal/adult tissues. Our detailed protocol for performing RNA in situ hybridization on Drosophila at later developmental time points. Format: DOC Size: 116KB Download file This file can be viewed with: Microsoft Word Viewer Additional file 8: Legends for supplemental Figures S1-S5. All supplemental figure titles and legends. Format: DOC Size: 35KB Download file This file can be viewed with: Microsoft Word Viewer |




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