Lola regulates Drosophila olfactory projection neuron identity and targeting specificity

doi:10.1186/1749-8104-2-14

Neural Development

Volume 2

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Su H
Giniger E
Komiyama T
Quake S
Luo L

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Research article

Lola regulates Drosophila olfactory projection neuron identity and targeting specificity

Maria Lynn Spletter¹ , Jian Liu²^,3 , Justin Liu¹ , Helen Su¹^,4 , Edward Giniger⁵ , Takaki Komiyama¹^,4 , Stephen Quake² and Liqun Luo¹

¹Howard Hughes Medical Institute, Department of Biological Sciences, Stanford University, Stanford, California 94305, USA

²Howard Hughes Medical Institute, Department of Bioengineering, Stanford University, Stanford, California 94305, USA

³Department of Biomedical Engineering, Emory University, Atlanta, Georgia 30322, USA

⁴Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA

⁵National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA

author email corresponding author email

Neural Development 2007, 2:14doi:10.1186/1749-8104-2-14


Published:	16 July 2007

Additional files

Additional file 1:

Additional lola mutant allele MARCM analysis demonstrating dendritic targeting defects. Supplemental Figure S1 showing targeting defects in lola^ore5D2, lola^orc4, and lola^ore119mutant PNs.

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Additional file 2:

Supplemental Tables S1-S6. Supplemental tables and legends referenced in the main text. This includes a semi-quantitative analysis of axon targeting defects in lola^-/-and lola^ORE5D20-/-, a complete summary of DL1 dendrite and axon phenotypes by allele as percentage affected, a complete summary of vPN dendritic phenotypes by allele as percentage targeted correctly, clonal frequencies for MARCM experiments and primer sequences for in situ hybridization probes.

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Additional file 3:

Additional lola isoform in situ in the Drosophila brain. Supplemental Figure S2 showing controls and verification for our in situ technique and additional lola isoform hybridization results.

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Additional file 4:

Additional lola isoform RT-PCR analysis in the Drosophila brain. Supplemental Figure S3 showing addition LCM RT-PCR experiments comparing lola isoform expression at different developmental time points and in different brain tissues.

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Additional file 5:

Effects of UAS-lola overexpression on adPN and lPN dendrites and axons. Supplemental Figure S4 showing adPN and lPN phenotypes in MARCM clones in a wild-type background expressing UAS-lola A, UAS-lola L and UAS-lola T.

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Additional file 6:

adNB and lNB phenotypes in lola^-/-, UAS-lola MARCM clones. Supplemental Figure S5 showing adPN and lPN phenotypes in lola^-/-clones expressing UAS-lola A, UAS-lola L and UAS-lola T.

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Additional file 7:

Protocol for RNA in situ hybridization in fly larval/pupal/adult tissues. Our detailed protocol for performing RNA in situ hybridization on Drosophila at later developmental time points.

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Additional file 8:

Legends for supplemental Figures S1-S5. All supplemental figure titles and legends.

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