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Open Access Research article

Lola regulates Drosophila olfactory projection neuron identity and targeting specificity

Maria Lynn Spletter1, Jian Liu23, Justin Liu1, Helen Su14, Edward Giniger5, Takaki Komiyama14, Stephen Quake2 and Liqun Luo1*

Author Affiliations

1 Howard Hughes Medical Institute, Department of Biological Sciences, Stanford University, Stanford, California 94305, USA

2 Howard Hughes Medical Institute, Department of Bioengineering, Stanford University, Stanford, California 94305, USA

3 Department of Biomedical Engineering, Emory University, Atlanta, Georgia 30322, USA

4 Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA

5 National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA

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Neural Development 2007, 2:14  doi:10.1186/1749-8104-2-14

Published: 16 July 2007

Additional files

Additional file 1:

Additional lola mutant allele MARCM analysis demonstrating dendritic targeting defects. Supplemental Figure S1 showing targeting defects in lolaore5D2, lolaorc4, and lolaore119 mutant PNs.

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Additional file 2:

Supplemental Tables S1-S6. Supplemental tables and legends referenced in the main text. This includes a semi-quantitative analysis of axon targeting defects in lola-/- and lolaORE5D20-/-, a complete summary of DL1 dendrite and axon phenotypes by allele as percentage affected, a complete summary of vPN dendritic phenotypes by allele as percentage targeted correctly, clonal frequencies for MARCM experiments and primer sequences for in situ hybridization probes.

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Additional file 3:

Additional lola isoform in situ in the Drosophila brain. Supplemental Figure S2 showing controls and verification for our in situ technique and additional lola isoform hybridization results.

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Additional file 4:

Additional lola isoform RT-PCR analysis in the Drosophila brain. Supplemental Figure S3 showing addition LCM RT-PCR experiments comparing lola isoform expression at different developmental time points and in different brain tissues.

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Additional file 5:

Effects of UAS-lola overexpression on adPN and lPN dendrites and axons. Supplemental Figure S4 showing adPN and lPN phenotypes in MARCM clones in a wild-type background expressing UAS-lola A, UAS-lola L and UAS-lola T.

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Additional file 6:

adNB and lNB phenotypes in lola-/-, UAS-lola MARCM clones. Supplemental Figure S5 showing adPN and lPN phenotypes in lola-/- clones expressing UAS-lola A, UAS-lola L and UAS-lola T.

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Additional file 7:

Protocol for RNA in situ hybridization in fly larval/pupal/adult tissues. Our detailed protocol for performing RNA in situ hybridization on Drosophila at later developmental time points.

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Additional file 8:

Legends for supplemental Figures S1-S5. All supplemental figure titles and legends.

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