Dendritic targeting defects in lola-/- MARCM clones. Representative confocal images of (a) wild-type control and (b) lola-/- anterodorsal (1), lateral (2), or ventral (3) MARCM neuroblast (NB) clones or DL1 single cell (SC) clones (4) labeled with Gal4-GH146, and anterodorsal (5) and lateral (6) neuroblast clones labeled with Gal4-Mz19. Blue arrows and circles demark a loss of correct targeting, while yellow arrows and circles demark off-target innervation. Both anterodorsal and ventral lola-/-clones have ectopic dendritic extensions to the suboesophageal ganglion (SOG). anti-CD8::GFP in green, anti-nc82 neuropil in magenta. Scale bar, 20 μm. (c1) Normal targets of Gal4-GH146 in anterior, middle and posterior sections of the AL. Red denotes dorsal target, blue denotes lateral target, and green denotes ventral target. (c2) Quantification of adNB, lNB, vNB and DL1 clone phenotypes. The percentage of clones targeting a particular glomerulus is denoted on the Y-axis, while individual glomeruli are listed on the X-axis and grouped according to lineage by color as in (c1). Glomeruli in orange are not normally targeted by Gal4-GH146 positive PNs by heatshocking at 24 h ALH. Control clone innervation is denoted by colored bars corresponding to lineage, and lola-/- clone innervation is denoted by black bars. Note the decrease of correct targeting and increase of off-target innervation in lola-/- brains for all clone types. Additionally, the final 'diffuse' bar quantifies the number of brains that exhibit diffuse and wandering dendrites that fail to specifically innervate glomeruli.
Spletter et al. Neural Development 2007 2:14 doi:10.1186/1749-8104-2-14