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Making sense of zebrafish neural development in the Minervois

Alain Ghysen1 email, Christine Dambly-Chaudière1 email and David Raible2 email

Lab. Neurogenetics, INSERM U881, Université Montpellier 2, 34095 Montpellier, France

Department of Biological Structure, University of Washington, Seattle, WA 98195-7420, USA

author email corresponding author email

Neural Development 2007, 2:15doi:10.1186/1749-8104-2-15

Published: 8 August 2007

Additional files


Additional file 1:

Migration of the posterior lateral line primordium as visualized in a claudin-GFP line. Movie provided by D Gilmour

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Additional file 2:

Migration of the posterior lateral line primordium in a cxcr7 morphant embryo. Movie provided by D Gilmour

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Additional file 3:

Migration of the posterior lateral line primordium in a cxcr4b mutant embryo. Movie provided by D Gilmour

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Additional file 4:

Time-lapse observation of a growing lateral line axon in a 28 hph embryo injected with the HuC-Kaede plasmid. Time-lapse observation of a growing lateral line axon in a 28hph embryo injected with the HuC-Kaede plasmid, as described in [7]. In this case, Kaede was not photoconverted. Movie by Hideomi Tanaka, Tomomi Sato and Hitoshi Okamoto.

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Additional file 5:

Time-lapse observation of the same lateral line axon displayed in three dimensions. Movie by Hideomi Tanaka, Tomomi Sato and Hitoshi Okamoto

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Additional file 6:

Second-order projection from the lateral line to the torus semicircularis. A series of 22 frontal planes, from posterior to anterior, taken from a 300 micron thick frontal vibratome section. The fibers enter as bundles (first frames) and arborize as they reach their targets. The LT branch arborizes along the torus semicircularis; as this branch reaches its most anterior extent (last frames) a few fibers escape and climb dorsally into the overlying tectum (yellow arrows). Also present in this figure is the PM branch that arborizes just anterior to the oculomotor nucleus. Movie provided by A Ghysen.

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Additional file 7:

Three-dimensional stack documenting the second-order projection illustrated Figure 8, from the most ventral to the most dorsal plane (13 planes, whole-mount preparation). The dorsal planes reveal a few fibers climbing dorsally from the anterior tip of the LT branch into the deep layer of the optic tectum (yellow arrows). Movie provided by A Ghysen.

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Additional file 8:

A blind Mexican cave fish leisurely swimming in an annular arena with Lego landmarks to which the fish is accustomed. This clip shows part of the last training trial (control) after the fish had been repeatedly presented with the experimental arena. Movie provided by T Burt de Perera.

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Additional file 9:

The same cavefish in the same arena, after two of the landmarks had been switched (test trial), changing their order. Note how much faster the fish is swimming in the test trial than in the control, indicating that it had recognized a change in its environment. Movie provided by T Burt de Perera.

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Additional file 10:

Individual efferent neuron in the optic tectum, stochastically labeled with the Cre-loxP/Gal4-UAS system. Individual efferent neuron in the optic tectum, stochastically labeled with the Cre-loxP/Gal4-UAS system described in [8]. Movie by Tomomi Sato and Hitoshi Okamoto.

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Additional file 11:

The fish startle response. The time of mechanical stimulation is shown on the lower line. Movie provided by Michael Granato.

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