HSPC300 and its role in neuronal connectivity

doi:10.1186/1749-8104-2-18

Neural Development

Volume 2

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Carrera P
Gautreau A
Schenck A
Giangrande A

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Research article

HSPC300 and its role in neuronal connectivity

Abrar Qurashi¹^,2 , H Bahar Sahin¹ , Pilar Carrera¹^,3 , Alexis Gautreau⁴ , Annette Schenck¹^,5 and Angela Giangrande¹

¹Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM/ULP, BP 10142, 67404 Illkirch, CU de Strasbourg, France

²Department of Human Genetics, Emory University School of Medicine, Atlanta, GA 30322, USA

³Abteilung für Molekulare Entwicklungsbiologie, Institut für Molekulare Physiologie und Entwicklungsbiologie, Universität Bonn, D-53115 Bonn, Germany

⁴Laboratoire de Morphogenèse et Signalisation Cellulaires, UMR 144 CNRS/Institut Curie, 75248 Paris Cedex 05, France

⁵Department of Human Genetics (855), Nijmegen Centre for Molecular Life Science, Radboud University Nijmegen Medical Centre, Box 9101, 6500 HB Nijmegen, The Netherlands

author email corresponding author email

Neural Development 2007, 2:18doi:10.1186/1749-8104-2-18


Published:	25 September 2007

Additional files

Additional file 1:

Molecular characterization of excision line HSPC300^Δ54.3. Sequence obtained upon 5' and 3' inverse PCR (compared with original line EP(2R)0506) showing the presence of intact junctions between the P element and surrounding genomic sequences. Note that, following 208 bases of the HSPC300 sequence, an unrelated sequence of at least 74 bases is present.

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Additional file 2:

Normal levels and localization of other WAVE/SCAR complex proteins (CYFIP, SCAR) in HSPC300 zygotic null embryos. (a-f) Wild-type (WT), HSPC300 zygotic null and, as control, CYFIP zygotic null embryos labeled with anti-CYFIP (a-c) or anti-SCAR (d-f) antibodies. (g) Anti-CYFIP and anti-SCAR immunoblot analysis of WT and HSPC300 zygotic null mutant extracts. Note that in contrast to genetic conditions in which maternal and zygotic HSPC300 doses have been depleted, there is no appreciable difference in CYFIP and SCAR levels and distribution in HSPC300 zygotic null embryos. Maternally provided HSPC300 protein is thus sufficient to stabilize other WAVE/SCAR complex proteins during embryonic development. In contrast, despite maternal contribution, loss of zygotic CYFIP destabilizes SCAR. Scale bar: 75 μm.

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