|
Respiratory parameters and anatomical measures of wild-type, heterozygous and homozygous Hoxa2 mutant mice at birth |
|||
| Wild-type |
Heterozygous |
Homozygous |
|
|
|
|||
| Respiratory and behavioral parameters at P0.1 |
|||
| N |
38 |
58 |
29 |
| Birth weight (g) |
1.45 ± 0.006 |
1.46 ± 0.022 |
1.40 ± 0.024 |
| Weight gain at P0.75 |
|||
| Grams |
0.24 ± 0.01 |
0.22 ± 0.01 |
-0.09 ± 0.008* |
| Percent |
16.5 ± 1.0 |
15.1 ± 0.9 |
-6.6 ± 0.6* |
| Jaw openings (in 30 s) |
24.0 ± 0.41 |
23.9 ± 1.22 |
13.6 ± 1.63* |
| fR (cycles/minute) |
107 ± 1.3 |
107 ± 4.0 |
110 ± 5.7 |
| Tidal volume (μl/g) |
4.6 ± 0.06 |
5.3 ± 0.41 |
10.6 ± 0.87* |
| VE (ml/g/minute) |
0.52 ± 0.01 |
0.58 ± 0.06 |
1.21 ± 0.14* |
| Apneas (% of recording duration) |
11 ± 0.8 |
9.6 ± 3.0 |
8.1 ± 3.7 |
| Anatomical measures |
|||
| N |
8 |
4 |
12 |
| AP length of locus coeruleus (μm) |
455 ± 18 |
420 ± 60 |
717 ± 23* |
| DV height of locus coeruleus (μm) |
625 ± 60 |
700 ± 20 |
1,017 ± 73† |
| Distance between LC ventral limit (μm) |
1,050 ± 25 |
Not measured |
607 ± 79* |
|
Values are given as mean ± standard error of the mean. fR, respiratory frequency; VE, minute ventilation. Tidal volume at birth was increased two-fold in Hoxa2-/- mice (see also Figure 2), but this feature disappeared rapidly: VE at P0.75 was not significantly different between the three genotypes. Note that Hoxa2-/- mice lost weight between birth and P0.75, while their wild-type and heterozygous littermates gained weight. The birth weight and respiratory frequency are not significantly different. *P < 0.001; †P < 0.01. DV, dorso-ventral; LC, locus coeruleus. | |||
Chatonnet et al. Neural Development 2007 2:19 doi:10.1186/1749-8104-2-19 |
|||