Metamorphosis of an identified serotonergic neuron in the Drosophila olfactory system

doi:10.1186/1749-8104-2-20

Neural Development

Volume 2

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Roy B
Singh AP
Shetty C
Chaudhary V
North A
Landgraf M
VijayRaghavan K
Rodrigues V

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Singh AP
Shetty C
Chaudhary V
North A
Landgraf M
VijayRaghavan K
Rodrigues V
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Research article

Metamorphosis of an identified serotonergic neuron in the Drosophila olfactory system

Bidisha Roy¹ , Ajeet P Singh² , Chetak Shetty² , Varun Chaudhary¹ , Annemarie North³ , Matthias Landgraf³ , K VijayRaghavan¹ and Veronica Rodrigues¹^,2

¹National Centre for Biological Sciences, TIFR, GKVK PO, Bangalore 560065, India

²Department of Biological Sciences, Tata Institute of Fundamental Research, Homi Bhabha Rd, Mumbai 400005, India

³Department of Zoology, University of Cambridge, Downing Street, Cambridge CB2 3EJ, UK

author email corresponding author email

Neural Development 2007, 2:20doi:10.1186/1749-8104-2-20


Published:	24 October 2007

Additional files

Additional File 1:

Broad expression pattern of RN2-Flp, Tub-FRT-CD2-FRT-Gal4, UAS-mCD8GFP in adult brain. (a) Large set of neurons marked as a result of flp activity in RN2-Flp, Tub-FRT-CD2-FRT-Gal4, UAS-mCD8GFP stock. (b) Both the CSD neurons (asterisk) have been marked (bilateral flipout). (c, d) A small set of central neurons (arrowheads). Cell body of the CSDn is indicated by an asterisk in (c). Scale bar = 30 μm.

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Additional File 2:

Localization of Dlg-GFP and Syt-GFP in the larval CSD neuron. (a, c) RN2-Flp, Tub-FRT-CD2-FRT-Gal4/UAS-Syt-GFP. (b, d) UAS-Dlg-GFP/+; RN2-Flp, Tub-FRT-CD2-FRT-Gal4. (a, b) Ipsilateral dendrites; (c, d) contralateral presynaptic terminals stained with anti-GFP (green) and mAbnc82 (red). Syt-GFP localizes to the cell body but does not extend beyond a few microns in the primary neurite (asterisk; (a, a')), but is enriched within the synaptic endings in the antennal lobe (dotted lines) appearing as punctate staining (c, c'). Dlg-GFP is localized mainly within the cell body and ipsilateral neurites (arrowheads in (b, b') and is very weakly present in the terminal arbors within the antennal lobe (dotted lines in (d, d'). (e, e') Syt-GFP shows punctate localization in the branches at ipsilateral (arrow) and contralateral higher centers (arrowhead in (e, e'). (f) Diagrammatic representation of Syt-GFP (green) and Dlg-GFP (red) expression. Dlg-GFP is enriched in the dendritic trees while Syt-GFP is localized to the presynaptic terminals of CSDn.

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Additional File 3:

Quantification of branch points. (a-d) Schematic of the branch point analysis of dendrites within the ipsilateral antennal lobe (a-c) and the contralateral terminals (d). (a, d) RN2-Flp, Tub-FRT-CD2-FRT-Gal4, UAS-mCD8GFP/+ dendritic (a) and terminal (d) arbors. (b) RN2-Flp, Tub-FRT-CD2-FRT-Gal4, UAS-mCD8GFP/UAS-EGFPKir2.1.(c) UAS-shi^ts1/+; RN2-Flp, Tub-FRT-CD2-FRT-Gal4, UAS-CD8GFP/UAS-shi^ts1. (e) Schematic showing that the neuron was imaged in 1 μm confocal sections through the brain. Each section was projected onto a monitor and the branch point denoted by a red dot on a cellulose acetate transparent sheet. The total number of branch points was counted over the whole z-stack.

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