Additional file 1.

Targeted delivery of EGFP expression plasmids by selective electroporation towards either the ventral neural tube or the neural crest. Confocal micrographs of transverse vibratome sections (75 μm). (a) An example of a HH23 chick embryo electroporated in the ventral neural tube with the pCA-EGFPm5-mU6 plasmid, as detailed in the Materials and methods. Motor neurons are labelled with a mixture of anti- MNR2 and Islet-2 antibodies (red). Note that virtually all islet-2/MNR2 positive motor neurons (MN) in this section appear yellow, indicating co-expression of EGFP. No EGFP expression is seen outside the neural tube. (b) The plasmid was targeted towards the dorsal neural tube. The section shown was taken several somites away from the electroporation site and shows EGFP expression in neural crest derivates only (dorsal root ganglion (DRG), sympathetic ganglia (Sn) and glia in ventral (VR) and dorsal (DR) spinal nerve roots). The anti-Islet-1,2 antibody (red) labels motor neurons, DRG neurons, sympathetic neurons and dorsal horn interneurons. Note the complete absence of EGFP expression within the spinal cord. Bar = 100 μm.

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Bron et al. Neural Development 2007 2:21   doi:10.1186/1749-8104-2-21