Additional file 2.

Npn-2 B and Npn-2 E shRNA reduce expression of V5-tagged cNpn-2 in HEK cells. Vectors co-expressing EGFP and shRNA targeted at Npn-2 were co-transfected with pcDNA3.1-V5-cNpn-2 [19] at a ratio of 5:1. At 2 days post-transfection, cells were fixed and stained with V5 antibodies. The data show efficient knockdown of V5-tagged Npn-2 with Npn-2 B and Npn-2 E shRNA. By contrast, the Npn-2 F shRNA construct is ineffective. Bar = 25 μm.

Format: PDF Size: 68KB Download file

This file can be viewed with: Adobe Acrobat Reader

Bron et al. Neural Development 2007 2:21   doi:10.1186/1749-8104-2-21