Additional file 3.

Screen of shRNA vectors targeting Npn or Plexin-A receptors in the chick for the ability to induce ectopic motor neuron migration. The prevalence of ectopic motor neurons after ventral electroporation of shRNA vectors targeting plexin-A and neuropilin receptors in the chick was assessed. In a first round of analysis, three different vectors were tested for each Plexin-A target along with validated npn-1 and Npn-2 shRNA vectors. After obtaining positive results for Npn-2B and Plexin-A2A shRNA, a second batch of triplicate vectors targeting Npn-2 and Plexin-A2 was tested. Of these vectors Npn-2 E and Plexin-A2D shRNA also induced ectopic motor neurons, whilst Npn-2 C, -2D, -2F and Plexin-A2E and -A2F constructs were ineffective. ***P < 0.001; two-tailed t-test.

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Bron et al. Neural Development 2007 2:21   doi:10.1186/1749-8104-2-21