Additional file 5.

Confirmation of knockdown of Plexin mRNAs by ventral targeted shRNA in the chick spinal cord. Embryos were electroporated at E2 (HH stage 13–15) in the ventral neural tube with shRNA vectors targeting (a,b) Plexin-A1, (c,d) Plexin-A2 or (e,f) Plexin-A4. The effect of each shRNA on expression was assessed after 48–54 hours (HH 23–24) by in situ hybridisation on transverse cryosections of hindlimb region spinal cord (20 μm) (b,d,f). Bar = 100 μm. The data show a clear reduction of the in situ signals of the targeted genes in the electroporated areas (enclosed by dashed lines), compared to corresponding regions on the un-electroporated, contralateral side.

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Bron et al. Neural Development 2007 2:21   doi:10.1186/1749-8104-2-21