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Transcripts differentially expressed in the DRG and TG |
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| Transcript |
Symbol |
Class |
Fold |
|
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| Greater expression in the DRG |
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| Homeobox B3 |
Hoxb32 |
TX |
(90.4) |
| Homeobox A9 |
Hoxa9 |
TX |
(90.2) |
| Homeobox A5 |
Hoxa52 |
TX |
(44.2) |
| Etv1; ER81 |
Etv13 |
TX |
(40.2) |
| Homeobox C8 |
Hoxc82 |
TX |
(38.4) |
| Homeobox B6 |
Hoxb6 |
TX |
(27.6) |
| Hoxd4 |
Hoxd4 |
TX |
(16.6) |
| Follistatin |
Fst2 |
Dev |
(12.9) |
| Leucine-rich repeat-containing G protein-coupled receptor 6 |
Lgr6 |
Other |
(11.8) |
| Receptor (calcitonin) activity modifying protein 2 |
Ramp23 |
ST |
10.9 |
| Ca+ channel, L type, alpha 1C subunit |
Cacna1c |
NT |
9.2 |
| Homeobox B8 |
Hoxb8 |
TX |
(9.1) |
| BTB and CNC homology 2 |
Bach2 |
TX |
(8.8) |
| Sclerostin domain containing 1 |
Sostdc1 |
Dev |
8.5 |
| Laminin, alpha 2 |
Lama2 |
Other |
(7.3) |
| Selected |
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| Purkinje cell protein 4 |
Pcp4 |
Unk |
(5.7) |
| LIM domain binding 2 |
Ldb22 |
TX |
5.6 |
| Zinc finger protein of the cerebellum 1 |
Zic12 |
TX |
5.3 |
| Gap junction membrane channel protein alpha 1 |
Gja12 |
NT |
5.1 |
| Protein tyrosine phosphatase, receptor type, E |
Ptpre3 |
ST |
5.0 |
| Early B-cell factor 1; Olf1 |
Ebf1 |
TX |
5.0 |
| U74 array |
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| Regulator of G-protein signaling 4 |
RGS4 |
NT |
6.1 |
| Endothelin receptor type B |
EDNRB |
Other |
3.0 |
| Anthrax toxin receptor 2 |
Antxr2 |
Unk |
3.0 |
| Greater expression in the TG |
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| Microfibrillar-associated protein 4 |
Mfap4 |
Unk |
(20.6) |
| Lectin, galactose binding, soluble 7 |
Lgals7 |
AX |
(11.3) |
| MyoD family inhibitor |
Mdfi |
TX |
(9.6) |
| Suppressor of cytokine signaling 3 |
Socs32 |
ST |
(6.4) |
| RNA imprinted and accumulated in nucleus |
Rian |
Unk |
6.1 |
| Musashi homolog 2 |
Msi2h |
Other |
6.0 |
| U74 array |
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| Neuropeptide Y receptor 1 |
NpyR1 |
NT |
8.6 |
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The principal analysis was conducted with the murine 430 array, with selected results identified using the U74A+B array set added. All listed transcripts showed increased expression (change p < 0.005) in two independent replicates. Fold values represent the ratio of the means of two determinations for each tissue. Fold changes in parentheses indicate an absent call, that is, below the statistically reliable limit of detection, in the lower expressing sample. Transcripts with expression levels less than 40% of the scaled mean are excluded. Superscript numerals represent the number of probe sets concordant for changed expression for a given gene. AX, axonogenesis; Dev, development; NT, neurotransmission; ST, signal transduction; Syn, synaptogenesis; TX, transcription; Unk, unknown. Greater expression in the DRG: confirmation of selected results by in situ hybridization and immunofluorescence appears in 1. Differential expression of Ramp2 could not be confirmed by ISH. Different levels of Zic1 and follistatin appear to be due to high expression in the spinal cord adjacent to the DRG, rather than the DRG itself. Sostdc1 and EDNRB showed increased DRG expression by ISH but in a pattern more characteristic of glial than neuronal expression, with signal concentrated in nerve roots. The expression of RGS4 in the developing sensory ganglia has been previously described [43], and its higher expression in the DRG at E13.5 appears to be due to the dynamic developmental regulation of this gene, rather than a persisting difference in expression between the DRG and TG. Greater expression in the TG: in situ hybridization confirmed some changes (see 1). However, Mfap4 was widely expressed in embryonic tissues. Socs3 ISH exhibited relatively greater expression in the TG, but overall expression levels were low. Differential expression of the supressor of cytokine signaling Socs3 in the TG and DRG has previously been noted [44]. | |||
Eng et al. Neural Development 2007 2:3 doi:10.1186/1749-8104-2-3 |
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