Electroporation-based methods for in vivo, whole mount and primary culture analysis of zebrafish brain development

doi:10.1186/1749-8104-2-6

Neural Development

Volume 2

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Electroporation-based methods for in vivo, whole mount and primary culture analysis of zebrafish brain development

Michael Hendricks¹^,2 and Suresh Jesuthasan¹^,2

¹Developmental Neurobiology Group, Temasek Life Sciences Laboratory, Research Link, National University of Singapore, 117604, Singapore

²Department of Biological Sciences, National University of Singapore, Singapore

author email corresponding author email

Neural Development 2007, 2:6doi:10.1186/1749-8104-2-6


Published:	15 March 2007

Additional files

Additional file 1:

Three-dimensional volume rendering of electroporated neurons. A volume rendering of a confocal stack of a live 3 dpf embryo electroporated with pHuC:GAL4/pUAS:EGFP

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Additional file 2:

Time lapse of commissural axons. Time lapse series (7.5 minutes/frame) of confocal z-projections of habenular commissural axons crossing in a 2.5 dpf embryo unilaterally electroporated with pHuC:GAL4/pUAS:EGFP

Format: AVI Size: 4MB Download file

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