Michael Hendricks and Suresh Jesuthasan*
Corresponding author: Suresh Jesuthasan firstname.lastname@example.org
Neural Development 2007, 2:6 doi:10.1186/1749-8104-2-6
(2007-05-28 10:48) Temasek Life Sciences Laboratory
A couple of clarifications:
1. It is important the embryos in agarose be covered with normal E3 medium for recovery
after electroporation, not Ringers solution.
2. Small bubbles should appear on the electrodes during pulsing. Large bubbles (big
enough to deform the embryos) or no bubbles indicate non-optimal voltage, unclean
electrodes, or something wrong in the circuitry. Voltage may need to be optimized
due to possible variation in equipment, connectors, etc.
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