Methodology
Electroporation-based methods for in vivo, whole mount and primary culture analysis of zebrafish brain development
Neural Development 2007, 2:6 doi:10.1186/1749-8104-2-6
Methodology
Methodology
Neural Development 2007, 2:6 doi:10.1186/1749-8104-2-6
important points in using this technqie
Michael Hendricks (2007-05-28 10:48) Temasek Life Sciences Laboratory
A couple of clarifications:
1. It is important the embryos in agarose be covered with normal E3 medium for recovery after electroporation, not Ringers solution.
2. Small bubbles should appear on the electrodes during pulsing. Large bubbles (big enough to deform the embryos) or no bubbles indicate non-optimal voltage, unclean electrodes, or something wrong in the circuitry. Voltage may need to be optimized due to possible variation in equipment, connectors, etc.
Competing interests
None declared
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