Table 1

Electroporation results at 48 hpf

Injection of 0.7 mg/ml each pHuc:GAL4/pUAS:EGFP into the lateral forebrain and ventricle was followed by two manually applied trains of five 1 ms pulses at the indicated voltage. Transfection levels were greatly decreased with voltages lower than 30 V. *Embryos were viewed dorsally under a compound fluorescence microscope and categorized according to extent of transgene expression: +++, similar to Figure 2a or higher; ++, intermediate levels; +, few enough cells to make out and count individually, fewer than 20. ^†Of 80 embryos electroporated in 2 experiments, 3 were killed by mechanical damage during removal from agarose and are not included.

Hendricks and Jesuthasan Neural Development 2007 2:6   doi:10.1186/1749-8104-2-6