Additional data file 2.

Histological analysis of conditional Sp8 mutant forebrains. Nissl stained coronal sections at different developmental stages, as indicated. (a-f) Wild type (Wt), (a' -f') cKO, (a'' -f'') cKO no midline (most severe phenotype observed). At E12.5, conditional Sp8 mutant tissue sections revealed a significant size reduction of the telencephalon and the morphological absence of the septum at rostral levels (a', a''). Caudally, the basal ganglia appear as a single eminence (b', b'') when compared to controls (b). At E15.5, cKO forebrains lacked the septum (c, c'). Due to an almost complete disgenesis of the midline, the lateral ventricles appear as a single aqueduct space (compare (c) with the area indicated by the asterisk in (c'')). At more caudal levels, there is no obvious difference between Sp8cKO and Sp8cKO no midline. However, when compared to wild-type embryos, the internal capsule of conditional mutants showed aberrant fiber bundles extending towards the basal telencephalon (arrows in (d, d', d'')). At E18.5, further reduction of the forebrain size and a variable dysgenesis of the midline were observed (e, e', e''). Sp8-deficient embryos typically lack a discernable corpus callosum (CC); instead, unilaterally probst bundles (PB) were apparent (e, e', e'').

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Zembrzycki et al. Neural Development 2007 2:8   doi:10.1186/1749-8104-2-8