UV laser mediated cell selective destruction by confocal microscopy

doi:10.1186/1749-8104-3-11

Neural Development

Volume 3

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Aigouy B
Asensio ML
Giangrande A

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UV laser mediated cell selective destruction by confocal microscopy

Laurent Soustelle , Benoît Aigouy , Marie-Laure Asensio and Angela Giangrande

Institut de Génétique et Biologie Moléculaire et Cellulaire, IGBMC/CNRS/ULP/INSERM – BP 10142 67404 ILLKIRCH, c.u. de Strasbourg, France

author email corresponding author email

Neural Development 2008, 3:11doi:10.1186/1749-8104-3-11


Published:	28 April 2008

Abstract

Analysis of cell-cell interactions, cell function and cell lineages greatly benefits selective destruction techniques, which, at present, rely on dedicated, high energy, pulsed lasers and are limited to cells that are detectable by conventional microscopy. We present here a high resolution/sensitivity technique based on confocal microscopy and relying on commonly used UV lasers. Coupling this technique with time-lapse enables the destruction and following of any cell(s) in any pattern(s) in living animals as well as in cell culture systems.