Additional file 2.Confocal assisted UV laser selective cell destruction. (a,b) Confocal images of repo::GFP expressing cells prior to (a) and after (b) selective destruction of glial cells in a developing Drosophila wing (indicated by the dotted area in (a,b)). Distal is to the right. (a) Cells targeted for selective destruction are indicated by arrows. (b) After UV irradiation (17 hours after puparium formation), GFP labeling rapidly faded, suggesting that targeted cells die (indicated by asterisks). (c) Repo immunolabeling on the same dissected wing at 22 hours after puparium formation. Cell death is confirmed by lack of Repo glial-specific labeling in the targeted cells (indicated by asterisks) whereas other glial cells (included in the dotted area) were not affected. Format: TIFF Size: 311KB Download file Soustelle et al. Neural Development 2008 3:11 doi:10.1186/1749-8104-3-11 |