Regulation of survival in adult hippocampal and glioblastoma stem cell lineages by the homeodomain-only protein HOP

Arianna De Toni^*¹ , Marie Zbinden^*² , Jonathan A Epstein³ , Ariel Ruiz i Altaba² , Alain Prochiantz¹^,4 and Isabelle Caillé¹^,5

¹UMR CNRS 8542, ENS, rue d'Ulm, 75005 Paris, France

²University of Geneva Medical School, 8542 CMU, rue Michel Servet, CH-1211 Geneva 4, Switzerland

³Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Curie Boulevard, Philadelphia, Pennsylvania 19104, USA

⁴Collège de France, place Marcelin Berthelot, 75231 Paris Cedex 05, France

⁵UFR de Biologie, Université Denis Diderot, 75013 Paris, France

author email corresponding author email* Contributed equally

Neural Development 2008, 3:13doi:10.1186/1749-8104-3-13


Published:	28 May 2008

Additional files

Additional file 1:

Proliferation of 3PGDH immunoreactive SGL cells. (a-c) After a 2 h pulse of BrdU, mice were processed for 3PGDH/BrdU immunocytochemistry. Some 3PGDH immunoreactive cells incorporate BrdU. (c) Higher magnification of merged (a) and (b) with orthogonal projections. Scale bar: 10 μm. (d) After a 2 h pulse of BrdU, mice were processed for 3PGDH/BrdU ICC and the double-labeled SGL cells were counted under a confocal microscope. The data are presented as the number of 3PGDH/BrdU cells per mm of SGL. The number of 3PGDH/BrdU cells is unchanged in the HOP knock out (KO) mice compared to the wild type (WT).

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Additional file 2:

HOP interacts with SRF in the hippocampus. Western blot with HOP antibody shows that HOP is present in hippocampal extracts (total) and efficiently co-immunoprecipitated with SRF (IP SRF).

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