Figure 2.

Expression patterns of a subset of genes up-regulated in ventral r4 of Phox2b^LacZ/LacZ embryos. (a-j) In situ hybridizations on transverse sections of ventral r4 of Phox2b^LacZ/+ (a, c, e, g, i) or Phox2b^LacZ/LacZ (b, d, f, h, j) E10.5 mouse embryos. DIG-labeled riboprobes recognizing Igfbp5 (a, b), Hes1 (c, d), Sfrp1 (e, f), Tcf3 (g, h) and Sox13 (i, j) mRNA were used. (k, l) Immunofluorescence with an antibody recognizing Sox13 on transverse sections of ventral r4 of Phox2b^LacZ/+(k) or Phox2b^LacZ/LacZ (l) E10.5 mouse embryos.