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Postnatal retinal cell genesis as quantified by thymidine birthdating and either immunofluorescent or histological identification of cell fate |
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| P0 |
P2 |
P4 |
P6 |
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|
|
|
|
|
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| Cell type |
IF |
Hist* |
IF |
Hist* |
IF |
Hist* |
IF |
Hist* |
|
|
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| Bipolar |
2.19 ± 1.45 |
6.51 |
3.98 ± 2.66 |
17.04 |
12.81 ± 6.16 |
31.81 |
16.01 ± 6.31 |
25.74 |
| Cone type |
1.67 ± 1.15 |
NA |
2.73 ± 1.90 |
NA |
7.96 ± 3.23 |
NA |
8.20 ± 2.97 |
NA |
| Rod type |
0.52 ± 0.32 |
NA |
1.21 ± 1.01 |
NA |
4.85 ± 3.35 |
NA |
7.82 ± 3.41 |
NA |
| Amacrine |
NA |
9.16 |
NA |
0.05 |
NA |
0.00 |
NA |
0.00 |
| Rod |
69.69 ± 12.65 |
81.67 |
85.73 ± 4.15 |
79.51 |
71.22 ± 5.88 |
59.54 |
62.74 ± 8.63 |
60.59 |
| Muller |
0.68 ± 0.26 |
2.66 |
0.58 ± 0.43 |
3.40 |
2.73 ± 1.08 |
8.65 |
4.04 ± 1.97 |
13.67 |
|
Postnatal mice were injected with tritiated thymidine at the developmental time indicated. For the immunofluorescent (IF) methodology, retinae were harvested at P16, dissociated and stained using established cell-type specific markers, namely anti-Chx10 (bipolar cells), Rho4D2 (rod photoreceptors), and anti-glutamine synthetase (Muller cells). *For the histochemical (Hist) estimates all data are based on [19]. Cells born from the central and peripheral sections were summed to thereafter derive a percentage of each cell type | ||||||||
Morrow et al. Neural Development 2008 3:2 doi:10.1186/1749-8104-3-2 |
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