 Research articleA chemical-genetic strategy reveals distinct temporal requirements for SAD-1 kinase in neuronal polarization and synapse formationJoanne SM Kim1,2 1 Department of Molecular Genetics, University of Toronto, Toronto, Ontario, M5S 1A8, Canada 2 Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, M5G 1X5, Canada 3 Department of Molecular and Cellular Biology and Center for Brain Science, Harvard University, Cambridge, MA 02138, USA 4 Howard Hughes Medical Institute and Department of Molecular and Cellular Pharmacology, University of California, San Francisco, CA 94143, USA
Neural Development 2008, 3:23doi:10.1186/1749-8104-3-23
Additional filesAdditional file 1: Experimental system and different exposures (1). Exposures to DMSO and/or 1NA-PP1. Each flow corresponds to a time-course as indicated in square boxes. L4 animals were exposed to DMSO/1NA-PP1, and their progenies were transferred to agar plates at different stages to be observed immediately or at an adult stage. Full lifetime exposures required four days of incubation; solutions were renewed after three days. Format: PDF Size: 21KB Download file This file can be viewed with: Adobe Acrobat Reader Additional file 2: Experimental system and different exposures (2). Exposures to DMSO and/or 1NA-PP1. Each flow corresponds to a time-course as indicated in square boxes. Gravid adult animals were sacrificed to obtain synchronized populations. Synchronized animals were exposed to DMSO/1NA-PP1 during different larval stages and observed immediately or at an adult stage. Format: PDF Size: 21KB Download file This file can be viewed with: Adobe Acrobat Reader |
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