 Research articleFak56 functions downstream of integrin alphaPS3betanu and suppresses MAPK activation in neuromuscular junction growthPei-I Tsai1,2 1 Institute of Molecular Biology, Academia Sinica, Taipei 115, Taiwan 2 Institute of Molecular Medicine, National Taiwan University, Taipei 106, Taiwan 3 Institute of Molecular Medicine, National Tsing Hua University, Hsinchu 300, Taiwan 4 Umeå Center for Molecular Pathogenesis, Umeå University, Umeå, S-901 87, Sweden 5 Department of Cell Biology and Program in Neuroscience, Harvard Medical School, Boston, Massachusetts 02115, USA 6 Institute of Biological Chemistry, Academia Sinica, Taipei 115, Taiwan 7 Indian Institute of Science Education and Research, 900, NCL Innovation Park, Homi Bhabha Road, Pune 411008, India
Neural Development 2008, 3:26doi:10.1186/1749-8104-3-26
Additional filesAdditional file 1: Fak56 mutant alleles and expression. This file describes (A) the Fak56 locus and the generation of N30 and K24 deletions from KG00304 P-element insertion, (B) the expression of Fak56 mRNA in different Fak56 allele combinations, (C, D) HRP staining for wild-type and Fak56CG1 NMJs, (E) quantifications for NMJ phenotypes, and (F) the knock-down effect of Fak56RNAi. Format: PDF Size: 357KB Download file This file can be viewed with: Adobe Acrobat Reader Additional file 2: Expressions of NMJ proteins in Fak56null. This file describes identical expressions of Dlg (A, B), Brp (C, D), dPak (E, F), GluIIA (G, H) and Fustch (I, J) at wild-type and Fak56N30/K24 NMJs. Format: PDF Size: 568KB Download file This file can be viewed with: Adobe Acrobat Reader Additional file 3: Ultrastructures of Fak56N30/K24 synapses. Electron micrographs of cross-sections through a type-I bouton of muscle 6/7 in wild-type (A) and Fak56N30/K24 (B) larvae. Quantitative analyses reveal no difference for synaptic unltrastructures (C). Format: PDF Size: 1.7MB Download file This file can be viewed with: Adobe Acrobat Reader Additional file 4: Electrophysiological recording of postsynaptic currents from wild-type and Fak56N30/K24 in 1 mM [Ca2+]. (A) Cumulative frequency plot reveals a significant shift in the distribution of mEJP amplitudes. (B) Representative traces and mean amplitudes of EJPs in wild-type and Fak56N30/K24. Format: PDF Size: 91KB Download file This file can be viewed with: Adobe Acrobat Reader Additional file 5: BMP/Gbb signaling-independent mechanism of Fak56 in NMJ growth. No alternations of NMJ phenotypes were detected by introducing mutant alleles (sax4, witA12 and med13) for BMP signaling components into elav>Fak56RNAi. Format: PDF Size: 69KB Download file This file can be viewed with: Adobe Acrobat Reader Additional file 6: ERK phosphorylation in Fak56CG1 mutant embryos. Expressions of phospho-ERK appear grossly normal during Drosophila embryogenesis. Format: PDF Size: 838KB Download file This file can be viewed with: Adobe Acrobat Reader |
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