Additional file 2.
Loss of F-actin in neural crest cells that received N19-rhoA or N19-rhoB. Neural tubes were electroporated in ovo with control green fluorescent protein (GFP) (A-C), N19-rhoA (D-F) or N19-rhoB (G-I). Neural primordia were then isolated and explanted. In all cases, transfected progenitors delaminated from neural tube (NTs) (A,A',D,D',G,G'). Control GFP+ neural crest (NC) cells exhibited actin+ stress fibers (arrows in (B,C)) but NC cells that received either N19-rhoA or N19-rhoB were devoid of stress fibers (arrows in (E,F) and (H,I)) when compared both to control-GFP and to untransfected cells in the same cultures. Some adopted irregular morphologies were also observed upon C3 and Y27632 treatments (E and inset). (A,D,G) Phase contrast. (A',B,D',E,G'H) GFP immunostaining. (C,F,I) Phalloidin. M, mesenchymal. Bar: 70 μM (A,A',D,D',G,G'); 5 μM (B,C,E,F,H,I).
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Groysman et al. Neural Development 2008 3:27 doi:10.1186/1749-8104-3-27