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Transient axonal glycoprotein-1 (TAG-1) and laminin-α1 regulate dynamic growth cone behaviors and initial axon direction in vivo

Marc A Wolman1,4 email, Vinoth K Sittaramane3 email, Jeffrey J Essner2,5 email, H Joseph Yost2 email, Anand Chandrasekhar3 email and Mary C Halloran1 email

1Departments of Zoology and Anatomy, and Neuroscience Training Program, University of Wisconsin, Madison, Wisconsin 53706, USA

2Department of Neurobiology and Anatomy, University of Utah School of Medicine, Salt Lake City, Utah 84112-5330, USA

3Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211, USA

4Department of Cell and Developmental Biology, University of Pennsylvania Medical School, Philadelphia, PA 19104, USA

5Department of Genetics, Development and Cell Biology, Iowa State University, Ames, IA 50011, USA

author email corresponding author email

Neural Development 2008, 3:6doi:10.1186/1749-8104-3-6

Published: 20 February 2008

Additional files


Additional file 1:

Time-lapse sequence of normal MLF axon outgrowth in an uninjected Tg(pitx2c:gfp) embryo. Ventral view, anterior to the left, ventral midline is up. The excerpt begins at 17 hpf. The movie shows MLF axons converging and MLF growth cones fasciculating along neighboring MLF axons. Images were captured every minute, and the movie runs at ten frames per second.

Format: MOV Size: 4MB Download file

Playing the movie within this page requires QuickTime and JavaScript. Read more


Additional file 2:

Time-lapse sequence of normal MLF axon outgrowth in an uninjected Tg(pitx2c:gfp) embryo. Ventral view, anterior to the left, ventral midline is up. The excerpt begins at 17 hpf. The movie shows a MLF growth cone (top left) extend into tissue outside the nucMLF zone, collapse, retract, and then fasciculate along an adjacent MLF axon. Images were captured every minute, and the movie runs at ten frames per second.

Format: MOV Size: 10MB Download file

Playing the movie within this page requires QuickTime and JavaScript. Read more


Additional file 3:

Time-lapse sequence of MLF axon outgrowth in a TAG1MO injected Tg(pitx2c:gfp) embryo. Ventral view, anterior to the left, ventral midline is up. The excerpt begins at 17 hpf. The movie shows MLF axons aberrantly growing into the tissue surrounding the nucMLF. Images were captured every minute, and the movie runs at ten frames per second.

Format: MOV Size: 7.2MB Download file

Playing the movie within this page requires QuickTime and JavaScript. Read more


Additional file 4:

Time-lapse sequence of MLF axon outgrowth in a bal;Tg(pitx2c:gfp) embryo. Ventral view, anterior to the left, ventral midline is up. The excerpt begins at 18 hpf. The movie shows MLF axons aberrantly innervating surrounding tissue and growing towards the ventral midline. Images were captured every minute, and the movie runs at ten frames per second.

Format: MOV Size: 8MB Download file

Playing the movie within this page requires QuickTime and JavaScript. Read more


Additional file 5:

Time-lapse sequence of MLF axon outgrowth in a TAG1MO injected Tg(pitx2c:gfp) embryo. Ventral view, anterior to the left, ventral midline is up. The excerpt begins at 17 hpf. The movie shows defective axon-axon interactions that precede MLF axon extension into surrounding tissues and alter neuronal polarity. Images were captured every minute, and the movie runs at ten frames per second.

Format: MOV Size: 10.2MB Download file

Playing the movie within this page requires QuickTime and JavaScript. Read more


Additional file 6:

Time-lapse sequence of MLF axon outgrowth in a bal;Tg(pitx2c:gfp) embryo. Ventral view, anterior to the left, ventral midline is up. The excerpt begins at 17.5 hpf. The movie shows MLF growth cones that maintain physical interactions with other MLF axons once contact is initiated. Images were captured every minute, and the movie runs at ten frames per second.

Format: MOV Size: 3MB Download file

Playing the movie within this page requires QuickTime and JavaScript. Read more


Additional file 7:

Time-lapse sequence of MLF axon outgrowth in a bal;Tg(pitx2c:gfp) embryo. Ventral view, anterior to the left, ventral midline is up. The excerpt begins at 18 hpf. The movie shows MLF growth cones that extend branches. Images were captured every minute, and the movie runs at ten frames per second.

Format: MOV Size: 6.9MB Download file

Playing the movie within this page requires QuickTime and JavaScript. Read more

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