Figure 2.

MLF growth cones fasciculate along neighboring MLF axons and are inhibited by surrounding tissue.(a, b) Images from time-lapse sequence of MLF axon outgrowth in uninjected Tg(pitx2c:gfp) embryos. Ventral views, anterior to the left, midline is up. Asterisks denote the caudal-most nucMLF cell. (a) The arrows indicate the position of the growth cone that fasciculates along a neighboring MLF axon. (b) The arrowheads label the growth cone that is repelled by surrounding tissue. The time stamp shows hours: minutes. Scale bar = 25 μm.