Figure 1.

RGC dendrites and putative glutamatergic postsynaptic sites visualized upon expression of td-Tomato and PSD95-YFP. (a) Whole-mount or en face view (top) and orthogonal projection (bottom) of a P7 bistratified RGC transfected with td-Tomato and PSD95-YFP. (b) Skeletonization of the cell, color-coded for depth. The ON sublamina is blue; OFF sublamina is orange. (c) Identification of PSD95-YFP puncta (cyan dots) by a semi-automated dotfinder program (see Figure 2). Td-Tomato dendritic fill is shown in red.