Dendrites differ from axons in patterns of microtubule stability and polymerization during development

Additional file 1:

Movement of EB-1 comets in a dendrite at 2 DIV. Recording of a portion of a dendrite (at 2 DIV) from Figure 1 shows EB-1 comets traveling in both anterograde and retrograde directions (the accompanying kymograph is shown in Figure 1B, C). Movie duration, 10 s, with frames (500 ms exposure) acquired in a continuous stream.

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Additional file 2:

Movement of EB-1 comets in a dendrite at 1 DIV. Segment of a dendrite (shown in Figure 6a') at 1 DIV showing most EB-1 comets traveling in the anterograde direction (toward the right), although retrograde events (toward the left) are clearly apparent as well.

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Additional file 3:

Movement of EB-1 comets in an axonal shaft at 1 DIV. Segment of axonal shaft (shown in Figure 6a") at 1 DIV. Both anterograde (downward) and retrograde (upward) events are clearly evident in this segment, and could be detected throughout the entire axon length.

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Additional file 4:

Movement of EB-1 comets in a dendritic arbor at 15 DIV. Dendritic arbor from a cell at 15 DIV. EB-1 comets can be detected throughout the arbor, traveling in both retrograde (toward the left) and anterograde (toward the right) directions, as well as entering a portion of dendritic spines.

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Additional file 5:

Movement of EB-1 comets in an axonal arbor at 15 DIV. Anterogradely oriented EB-1 comets could also be observed throughout the axonal arbor at 15 DIV, although with less frequency than was evident in younger cells. This recording was taken from the middle region of an axon, with the cell body to the right, and growth cone to the left.

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Additional file 6:

Recording from the distal region and growth cone at 15 DIV. Recording from the distal region, and growth cone (from the same cell as in Additional file 5) at 15 DIV.

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Additional file 7:

Movement of EB-1 comets in an axon at 3 DIV. Accompanying movie of the 3-DIV axon shown in Figure 8A, B. Anterograde events are evident throughout the shaft of the axon, as well as the branches and filopodia that arise from the shaft.

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Additional file 8:

Movement of EB-1 comets in a dendritic arbor at 3 DIV. Accompanying movie of the 3-DIV dendritic arbor shown in Figure 8D, E. Both anterograde and retrograde events throughout the length of the arbor are shown.

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Additional file 9:

Recording from a PC12 cell differentiated to take on a neuron-like phenotype. Both anterograde and retrograde events were observed in these cells, in about the same proportions as were observed in the dendritic arbors of more mature neurons.

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