Dorsal root ganglia (DRG) express Homer 1b/c, TRPC1 and STIM1 proteins in functionally relevant areas of growth cones in a pattern of close apposition and/or co-localisation. (A) Western blot analysis of DRG tissue probed with Homer1b/c (H1b/c), TRPC1 and STIM1 antibodies. (B, D) Immunostaining of DRG growth cones revealed a punctate expression pattern for Homer1b/c (B, red) and TRPC1 (D, green). (C, E) Immunostaining revealed a punctate expression pattern for Homer1b/c (C, red) and STIM1 (E, green) in DRG growth cones. (F, G) Merged red/green images show close association of Homer1b/c with TRPC1 (F) and STIM1 (G). (H, I) Analysis of colocalisation probability depicted by pseudo-coloured images (white = high probability; blue = low probability) underscores the close apposition of Homer1b/c, TRPC1 and STIM1 proteins in functionally relevant areas of growth cones such as central area (arrows) and distal filipodial tips (arrowheads). Scale bar: 5 μm.
Gasperini et al. Neural Development 2009 4:29 doi:10.1186/1749-8104-4-29