Figure 2.

Adult brain weight from Gpc1 null mutant is significantly reduced. (A) Dorsal views of freshly dissected adult brains. Note the apparent reduction in size of Gpc1^-/- brains. (B, C) Wet weights of fresh adult brains. Gpc1 genotypes are as indicated. There is a 15.5% decrease in brain weight in homozygous Gpc1 mutants (P < 0.0001); heterozygotes show an intermediate phenotype (7.8% decrease; * and **P < 0.0001). (B) N = 32 for +/+; 29 for +/-; 36 for -/-. Reduced brain weight is also observed in Gpc1^LacZ mutants. (C) N = 9 for +/+; 10 for LacZ/+; 7 for LacZ/LacZ. (D) Body weights of adult Gpc1 mutant and wild-type littermates, grouped by sex. No significant effect of genotype is observed. N = 23 for +/+; 30 for +/-; 37 for -/-. (E) Total DNA content of mutant and wild-type brains was measured as an indication of cell number. The data imply a 20% reduction in Gpc1^-/- brains. (*P < 0.001; N = 5 for +/+, and 5 for -/-).