Figure 2.

The pattern of Nolz1 protein expression resembles that of Nolz1 mRNA in the LGE. (A) Western blot analysis of striatal samples from different developmental stages showing high levels of Nolz1 protein at embryonic stages, which decrease during postnatal development. Within adult mice, Nolz1 expression remains at low levels in the striatum (Str) but is not detectable in the subependymal zone (SEZ). (B) In situ hybridization shows high levels of Nolz1 mRNA in the SVZ of the E14.5 vLGE. Note that the levels of Nolz1 mRNA decrease in the mantle zone (MZ). Scale bars: 600 μm. (C) Low levels of Nolz1 mRNA are also detected in the adult striatum but not in the subependymal zone. Scale bar: 150 μm. (D) Double in situ immunohistochemistry shows that within the MZ, Nolz1 expression is located in β-III tubulin (Tuj-1)-positive cells. Scale bar: 150 μm. (E) High magnification of (D). Scale bar: 60 μm.