Figure 2.

Zac1 is expressed in subsets of Golgi cells and Purkinje cells in the cerebellar cortex. (A-G) Expression of Zac1 in sagittal sections of the cerebellum at E16 (A), E18 (B), P0 (C), P3 (D), P8 (E), P12 (F) and P20 (G). Zac1⁺ cells in the granular layer are derived from the EGL. (H-K) Sagittal sections through E18 (H), P0 (I), P8 (J) and P20 (K) cerebella immunolabeled for calbindin (CaBP, green) and Zac1 (red), focusing on lobule IX. (L) A transverse section cut through the posterior cerebellum immunoperoxidase-stained at E18 with anti-Zac1. (M) A sagittal section through the cerebellum at E18 labeled with anti-Zac1 (red) and anti-BrdU (green; BrdU was injected into the pregnant dam 8 hours before the perfusion). Scale bars: 250 μm (G); 25 μm (H); 125 μm (L). EGL, external granular layer; GL, granular layer; IGL, internal granular layer; ML, molecular layer; PCL, Purkinje cell layer.