otd is expressed in PPL2 dopaminergic neurons in the adult brain. (A) otd (blue) expression in the PPL2 cell cluster of TH-positive (red) DA neurons in the adult brain. The PPL2 cell cluster labeled with a yellow asterisk is magnified and otd expression (A1-A4) and oc2 enhancer activity (A'1-A'4) are analyzed in single optical sections. Scale bars: 10 μm. (B) In ocγa1 hemizygous flies, the seven PPL2 DA neurons are not detected using anti-TH immunoreactivity. (C) Targeted expression of an otd-specific RNA interference construct in DA neurons (using the TH-gal4 driver) impairs the viability of four PPL2 DA neurons in the adult brain of otdYH13 heterozygous flies (7 days old). (C,C1-C3) TH and otd expression in the resulting PPL2 cluster (labeled with a yellow asterisk in (C)) is analyzed at higher magnification. Scale bars: 10 μm. (D1-D4) Targeted expression of P35 in Otd-depleted DA neurons (using the TH-gal4 driver) rescues cell viability and TH expression in PPL2 DA neurons in the adult brain of otdYH13 heterozygous flies (7 days old). Pictures represent single optical sections. Scale bars: 10 μm. (D'1-D'4) Otd protein levels in four PPL2 DA neurons (1, 2, 5 and 7) are not recovered. Panels (A-C,C1-C3) represent Z projections of individual confocal optical sections. Scale bars in (A-C): 50 μm. DA, dopaminergic; GFP, green fluorescent protein; oc, ocelliless; otd, orthodenticle; PPL, protocerebral posterior lateral; RNAi, RNA interference; TH, tyrosine hydroxylase.
Blanco et al. Neural Development 2011 6:34 doi:10.1186/1749-8104-6-34