Figure 2.

The centriole does not accumulate γ-tubulin in developing and mature Drosophila neurons. (A) Images of developing aCC and RP2 motor neurons expressing tau-LacZ and stained for γ-tubulin were taken in stage 12 embryos. Concentrated γ-tubulin was not seen in the neurons but was seen in distinct puncta in the surrounding epithelial cells (arrows), indicating the efficacy of the antibody. (B) Stage 16 embryos were stained with fluorescein isothiocyanate (FITC)-conjugated HRP and γ-tubulin antibody. Chordotonal neurons had γ-tubulin localization at the base of each cilium (red box), while no specific localization was seen in non-ciliated neurons. (C) Syncytial embryos were stained with γ-tubulin antibody. Dividing cells had γ-tubulin staining concentrated at the centrosome/spindle pole (arrows) and microtubules (between arrows) during metaphase. (D) Localization of both γ-tubulin-GFP and RFP-Fzr in third instar larvae is shown. Top: brains from larvae expressing γ-tubulin-GFP were examined after fixation. Concentrated areas of γ-tubulin-GFP localization (green arrows) were seen at spindle poles in dividing neuroblasts and show co-localization with RFP-Fzr (red arrows). Bottom: neurons in living larvae expressing γ-tubulin-GFP and RFP-Fzr were imaged. γ-Tubulin-GFP was diffuse throughout the neurons compared to RFP-Fzr, which was localized in a concentrated area (red arrow).