Figure 3.

Bap55 mutants cause derepression of a PN-GAL4. (A) WT Mz19 anterodorsal neuroblast clones label PNs targeting to the VA1d and DC3 glomeruli. The DC3 glomerulus is difficult to visualize in confocal stacks. (B) WT Mz19 lateral neuroblast clones label a single class of PNs targeting to the DA1 glomerulus. (C) WT Mz19 never labels ventral PN neuroblast clones. No labeling is observed in the antennal lobe. (D) Bap55^-/- Mz19 anterodorsal neuroblast clones exhibit ectopic labeling of PNs targeting many glomeruli in the anterior antennal lobe. (E) Bap55^-/- Mz19 lateral neuroblast clones exhibit ectopic labeling of local interneurons, which target throughout the antennal lobe. (F) Bap55^-/- Mz19 clones ectopically label ventral PNs, which are never labeled in WT, and that target to very few glomeruli in the antennal lobe. (G, H) WT Mz19 never labels a single anterodorsal PN when clones are generated just after larval hatching. (G₁, G₂) show no labeling in the antennal lobe. (H) shows no axon labeling in the lateral horn. (I, J) Bap55^-/- Mz19 exhibits ectopic labeling of single anterodorsal PNs, which target to the anterodorsal glomerulus DA4l (I₁), while avoiding the posterior glomerulus DL1 (I₂). The axons form an L-shaped pattern in the lateral horn, with branches in the mushroom body calyx (J). Green marks mCD8-GFP-labeled PNs generated by MARCM and labeled using Mz19-GAL4. (G, I) show partial confocal stacks; (A-F, H, J) show full confocal stacks; magenta is the presynaptic marker nc82; symbols are as in Figure 2. Scale bars: 20 μm in (A) (for A-G, I) and (H) (for H, J).