Figure 7.

E(Pc) mutants yield similar phenotypes to Bap55 mutants. (A, B) E(Pc)^-/- DL1 PNs mistarget their dendrites to the anterior glomerulus DA4l (A₁), avoiding the posterior glomerulus DL1 (A₂), and maintaining the stereotypical L-shaped axon targeting the lateral horn with branches in the mushroom body calyx (B). (C, D) Postmitotic expression of UAS-Bap55 causes a de novo phenotype in E(Pc)^-/- DL1 PNs. The dendrites split between DA4l and the anterior medial glomerulus DM6 (C₁), and the axon mistargets ventrally to the lateral horn (white arrow in (D)). (E) Quantification of mistargeting phenotypes for (A, C). Green marks mCD8-GFP-labeled PNs generated by MARCM and labeled using GH146-GAL4. (A, C) show partial confocal stacks; (B, D) show full confocal stacks; magenta is the presynaptic marker nc82; symbols are as in Figure 2. Scale bars: 20 μm in (A) (for A, C) and (B) (for B, D).