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Pannexin 1 regulates postnatal neural stem and progenitor cell proliferation

Abstract

Background

Pannexin 1 forms ion and metabolite permeable hexameric channels and is abundantly expressed in the brain. After discovering pannexin 1 expression in postnatal neural stem and progenitor cells we sought to elucidate its functional role in neuronal development.

Results

We detected pannexin 1 in neural stem and progenitor cells in vitro and in vivo. We manipulated pannexin 1 expression and activity in Neuro2a neuroblastoma cells and primary postnatal neurosphere cultures to demonstrate that pannexin 1 regulates neural stem and progenitor cell proliferation likely through the release of adenosine triphosphate (ATP).

Conclusions

Permeable to ATP, a potent autocrine/paracine signaling metabolite, pannexin 1 channels are ideally suited to influence the behavior of neural stem and progenitor cells. Here we demonstrate they play a robust role in the regulation of neural stem and progenitor cell proliferation. Endogenous postnatal neural stem and progenitor cells are crucial for normal brain health, and their numbers decline with age. Furthermore, these special cells are highly responsive to neurological injury and disease, and are gaining attention as putative targets for brain repair. Therefore, understanding the fundamental role of pannexin 1 channels in neural stem and progenitor cells is of critical importance for brain health and disease.

Background

The majority of neurons are born embryonically, however neurogenesis continues in the postnatal brain throughout life (reviewed in [1]). Postnatal neurogenesis is a complex, multi-step developmental process that includes cell behaviors such as proliferation, differentiation and migration, and involves several distinct cell types. These stem cell behaviors are guided, in part, by extracellular stimuli unique to their specialized microenvironments or ‘niches’: the subgranular zone (SGZ) of the dentate gyrus and the ventricular zone (VZ) of the lateral ventricles (Figure 1A).

Figure 1
figure 1

Panx1 is expressed in nestin-positive periventricular NSC/NPCs throughout postnatal development in vivo. (A) Mouse brain schematic with the neurogenic ventricular zone (VZ) and subgranular zone (SGZ) regions highlighted (i). Cell types throughout neurogenesis and the lineage markers expressed at each stage. Relatively quiescent NSCs become highly proliferative NPCs, and neuronally committed migratory neuroblasts before exiting the cell cycle as immature neurons (ii). Schematic of cell types in the periventricular region. The ependymal cell layer is interdigitated by thin processes or whole luminal surfaces of the radial glia-like NSCs (iii). This figure is a partial reproduction of another figure created by the same authors that appears in [2] and is reproduced with permission. (B) Western blot of lysates from HEK293T cells overexpressing EGFP, Panx1EGFP, or Panx1, showing specificity of the Panx1 Cterm antibody. (C) Confocal images of murine brain slices at the periventricular region. Nestin-positive NSCs exhibit high levels of Panx1 co-expression in postnatal day 15 (P15) (i), P30 (ii) and P60 (iii) brains. Arrows indicate areas of co-expression. (D) Image from a confocal z-stack with orthogonal side-views of a P30 periventricular region. Panx1 expression is noted in ependymal/sub-ependymal layers. (E) Confocal image from a P15 periventricular region. Panx1 is absent from DCX-positive neuroblasts. Hoechst 33342 was used as a nuclear counterstain in C-E. All scalebars 10 μm. DCX, doublecortin; NPC, neural progenitor cells; NSC, neural stem cells; Panx1, pannexin 1; V, ventricle.

Postnatal NSCs in the VZ generate large numbers of olfactory bulb interneurons. The distinct neural stem and progenitor cell (NSC/NPC) types implicated in this neurogenic process are characterized by their morphology and differential expression of lineage markers (Figure 1A). Radial glia-like cells (known as type B cells in VZ) express the filament markers nestin and glial fibrillary acidic protein (GFAP) (as well as several other lineage markers). Electron microscopy has shown that the ependymal layer lining the ventricles is not entirely contiguous and that type B cells make direct contact with the ventricle either via a large luminal surface or a thin cellular process (Figure 1A) [38]. These cells subsequently generate highly proliferative intermediate, transit-amplifying NPCs (C cells) that lose GFAP expression. Finally, further differentiation gives rise to migratory neuroblasts (A cells), which are committed to a neuronal lineage and express doublecortin (DCX). Following exit of the cell cycle, the immature neurons express class III beta tubulin, also known as Tuj-1.

Pannexins (Panx) are four-pass transmembrane proteins that oligomerize to form large pore ion and metabolite-permeable channels. Building on accumulating evidence that the coordinated activity of ion channels plays a major role in all aspects of NSC/NPC biology [9], we recently found that intracellular Panx2 modulates neuronal differentiation by impacting on neurite outgrowth and the timing of NSC/NPC commitment to a neuronal lineage [10].

Another Panx family member, Panx1, is also widely expressed in the brain as well as being found in the periphery [1115]. Panx1 forms large hexameric pores at the plasma membrane and/or at the endoplasmic reticulum membrane, depending on the cell type, and is permeable to ions and ATP in neurons, astrocytes and microglia [1621]. Purinergic nucleotides act as proliferation signals for NSC/NPCs, serving as negative regulators of terminal neuronal differentiation [2225]. NSC/NPCs express P2Y and P2X7 receptors and release ATP in episodic burst events, which act in an autocrine and paracrine manner to induce proliferation and negatively regulate differentiation. We have discovered Panx1 expression in postnatal NSC/NPCs. As large pore channels that mediate ATP release, we hypothesized that Panx1 channels might be positively associated with NSC/NPC proliferation.

Here we combine studies in the Neuro-2a (N2a) murine neuroblastoma-derived cell line as model NSC/NPCs, and VZ NSC/NPCs in vitro and in vivo, to elucidate the role of Panx1 in postnatal neuronal development. We detected Panx1 in nestin-positive and GFAP-positive NSC/NPCs and Tuj1-positive immature neurons, but not in intermediate DCX-positive neuronally committed neuroblasts in vitro and in vivo. Panx1 mediated ATP release, and blocking P2 purinergic receptors reduced cell proliferation, suggesting a possible role for Panx1 in regulating cell proliferation. Finally, by modulating Panx1 expression and activity in N2a cells and VZ neurosphere cultures, we demonstrated that Panx1 regulates NSC/NPC proliferation.

Results

Panx1 is expressed in periventricular NSC/NPCs

Using confocal immunofluorescence microscopy, we examined coronal cryosections of the postnatal periventricular area ranging from postnatal day 15 (P15) to P60 with lineage-marker antibodies (Figure 1C) and for Panx1 expression with an antibody (anti-Panx1 Cterm rabbit polyclonal, Invitrogen/Life Technologies, Burlington, Ontario, Canada) that we had previously characterized by immunoblotting of lysates from human-embyonic kidney (HEK) 293T cells transfected with Panx1, Panx1EGFP and EGFP (Figure 1B). Panx1 was detected in a sub-population of nestin-positive/GFAP-positive type B cells and nestin-positive type C cells in the ependymal/sub-ependymal layer of both the dorsal (Figure 1C, D) and ventral (not shown) aspects of the lateral ventricles. Panx1 was absent from DCX-positive committed neuroblasts (Figure 1E). These results suggest that Panx1 is expressed in periventricular NSC/NPCs and is lost upon neuronal commitment.

Panx1 mediates ATP release from NSC/NPCs

To further study the properties and cell biology of Panx1 in neuronal development we moved to in vitro culture models. We first employed the N2a cell line to examine the ability of Panx1 expression to influence ATP release from NPCs and to confirm the effects of blocking P2 receptors on NPC proliferation. The N2a cell line, derived from a murine neuroblastoma, is a widely used NPC model as these cells can be differentiated in the presence of retinoic acid and low serum [10, 26, 27]. N2a cells express endogenous Panx1 (Figure 2A). Panx1 is known to be activated by elevated K + [28, 29], which can rise above resting levels as much as 5 mM during periods of intense neuronal activity, up to more than 20 mM during injury and even higher during waves of spreading depression (reviewed in [30]). We treated N2a cells with varying concentrations of extracellular KCl, and found that ATP release was stimulated by elevating KCl concentrations (20 mM) compared to control (5.33 mM) and 0 mM KCl (P < 0.01 for one-way analysis of variance (ANOVA) with Tukey post-hoc, N = 3) (Figure 2B). Furthermore, treating the cells with the Panx1 blocker probenecid, which selectively blocks Panx1 hemichannels, and not connexin hemichannels [31], significantly decreased ATP release (60.50 ± 7.913% of control, P = 0.0159 for non-parametric t test) from proliferating N2a cells when compared to controls (Figure 2C). This is in accordance with the reported role of Panx1 in mediating ATP release in numerous other cell types (reviewed in [17]). The remaining ATP release could possibly be mediated by a vesicular release mechanism [32] or by connexin hemichannels ([33], but see also [34]). Finally, blocking Panx1 with probenecid (24 hours: control = 6.9 × 104 ± 6.4 × 103 cells, probenecid = 4.1 × 104 ± 1.8 × 103 cells, P < 0.001; 48 hours; control = 1.1 × 105 ± 4.8 × 103 cells, probenecid = 5.6 × 104 ± 4.7 × 103 cells, P < 0.001 for one-way ANOVA with Tukey post-hoc), or P2 receptors with pyridoxalphosphate-6-azophenyl-2′,4′-disulfonic acid (PPADS) [34], which blocks several P2X and P2Y isoforms [3542], significantly reduced N2a cell proliferation (24 hours: control = 6.9 × 104 ± 6.4 × 103 cells, PPADS = 4.6 × 104 ± 5.3 × 103 cells, P < 0.05; 48 hours: control = 1.1 × 105 ± 4.8 × 103 cells, PPADS = 8.1 × 104 ± 4.4 × 103 cells, P < 0.001 for one-way ANOVA with Tukey post-hoc) in accordance with the previously described role of P2 receptors in regulating NPC proliferation (Figure 2D) [2225].

Figure 2
figure 2

Panx1 blockage decreases ATP release from N2a cells and blocking P2 receptors reduces cell proliferation. (A) Panx1 is endogenously expressed in N2a cells, as demonstrated by RT-PCR (i) and western blot analysis (ii). To the left of the western blot, the asterisk (*) above the arrow denotes high-molecular weight species likely representing various Panx1 oligomeric forms, while the asterisk below the arrow denotes low molecular weight Panx1 cleavage products consistent in size with those reported in the literature [16]. (B) ATP release from N2a cells was stimulated upon treatment with 20 mM KCl buffer compared to control (5.33 mM) and 0 mM KCl conditions ((**) P < 0.01 for one-way ANOVA with post-hoc Tukey’s multiple comparison test, N = 3). (C) Blockage of Panx1 channels with 0.5 mM probenecid significantly decreases stimulated ATP release from N2a cells compared to controls (60.50 ± 7.913% and 99.17 ± 6.831%, respectively, N = 5, P = 0.0159 for non-parametric t test). (D) N2a cell numbers were quantified at 0, 24 and 48 hours for cells treated with 30 μM PPADS, 1 mM probenecid, or vehicle control. PPADS and probenecid treatments significantly reduced N2a numbers at 24 and 48 hours ((*) P < 0.05 and (***) P <0.001 for one-way ANOVA with post-hoc Tukey’s multiple comparison test, N = 3). ANOVA, analysis of variance; Panx, pannexin1, PPADS, pyridoxalphosphate-6-azophenyl-2′,4′-disulfonic acid.

Panx1 plays a major role in regulating VZ NSC/NPC proliferation

Given that Panx1 mediates ATP release in N2a model NPCs and that blocking P2 purinergic receptors inhibits proliferation, as shown in Figure 2, we hypothesized that Panx1 regulates NSC/NPC proliferation. To investigate the effect of Panx1 on N2a cell proliferation we overexpressed Panx1, and conversely, blocked Panx1 channels with probenecid [31]. Expression of Panx1EGFP, which localizes mainly at the plasma membrane in N2a cells (Figure 3A), as was previously reported in HEK293T cells [43], reduced the doubling time of N2a cells from 26.2 hours to 14.7 hours (cell counts increased by approximately 50%, P < 0.01 for one-way ANOVA with Tukey post-hoc, at 72 hours P < 0.001 for one-way ANOVA with Tukey post-hoc, N = 3) (Figure 3B) suggesting a positive effect of Panx1 on proliferation. Similarly, probenecid significantly reduced the proliferation of control EGFP expressing N2a cells, which endogenously express Panx1 (from 1.2 × 105 ± 1.1 × 104 cells to 4.0 × 104 ± 4.9 × 103 cells, P < 0.01 for one-way ANOVA with Tukey post-hoc, N = 3), as well as Panx1EGFP overexpressing cells (from 1.8 × 105 ± 2.4 × 104 cells to 6.0 × 104 ± 8.0 × 103 cells, P < 0.001 for one-way ANOVA with Tukey post-hoc, N = 3) (Figure 3C).

Figure 3
figure 3

Panx1 regulates N2a cell proliferation. (A) Image from a confocal z-stack with orthogonal side-views of N2a cells overexpressing Panx1EGFP. Panx1 is highly localized to the plasma membrane, stained with wheat germ agglutinin (WGA), as well as to intracellular membranes. (B) N2a cells overexpressing Panx1 exhibited a reduced doubling time (14.7 hours) compared to control cells overexpressing EGFP (26.2 hours) ((*) P < 0.05 and (**) P < 0.01 for one-way ANOVA with post-hoc Tukey’s multiple comparison test, N = 3). (C) Treatment of transfected N2a cells with the Panx1 channel blocker, probenecid (1 mM), significantly reduced cell proliferation at 48 hours in both Panx1 and EGFP overexpressing cells ((**) P < 0.01 and (***) P < 0.001 for one-way ANOVA with post-hoc Tukey’s multiple comparison test). ANOVA, analysis of variance; Panx1, pannexin1.

To determine whether Panx1 also regulates the proliferation of primary NSC/NPCs, we created neurosphere cultures from neonatal mice (P0 to P3), as previously described [10, 44] (Figure 4A). Panx1 mRNA and protein were expressed in VZ (and SGZ) derived neurosphere cultures, maintained for seven days in vitro (DIV), as assessed by RT-PCR and western blotting (Figure 4B). Confocal immunofluorescence microscopy of VZ neurosphere cultures with lineage markers confirmed Panx1 expression in nestin-positive/GFAP-positive NSCs and nestin-positive/GFAP-negative NPCs (Figure 4C). Furthermore, we plated VZ neurospheres on poly-D-lysine in the absence of mitogenic growth factors to induce differentiation, in order to investigate whether Panx1 is expressed in neuronally committed DCX-positive neuroblasts or Tuj-1-positive immature neurons by confocal immunofluorescence microscopy. As we observed in vivo, Panx1 immunoreactivity was absent in DCX-positive neuroblasts cultured under these neuronal driving conditions (Figure 4D). Finally, Panx1 was detected in Tuj-1 positive immature neurons (Figure 4E), consistent with previously reported neuronal Panx1 expression [10, 45].

Figure 4
figure 4

Panx1 expression in NSC/NPCs is re-capitulated in vitro in neurosphere cultures. (A) Outline of neurosphere culture generation from P0 to P3 hippocampus or microdissected VZ. Spheres are cultured for seven days in vitro (DIV) with addition of growth factors (hEGF and bFGF) every two DIV, before harvesting for subsequent analyses, or differentiation by removal of growth factors and plating on a poly-D-lysine coated surface. (B) Panx1 mRNA (i) and protein (ii) are expressed in VZ and SGZ neurospheres. To the left of the western blot, the asterisk (*) above the arrow denotes high-molecular weight species likely representing various Panx1 oligomeric forms, while the asterisk below the arrow denotes low molecular weight Panx1 cleavage products consistent in size with those reported in the literature [16]. (C) Confocal images showing Panx1 expression in a cryosectioned undifferentiated VZ neurosphere (i) and a digitally zoomed z-stack with orthogonal side-views (ii) in nestin-positive/GFAP-positive and nestin-positive/GFAP-negative cells. (D) Image from a confocal z-stack with orthogonal side-views of Panx1 expression in DCX-negative cells in a differentiated VZ neurosphere. (E) Image from a confocal z-stack with orthogonal side-views showing high levels of Panx1 co-expression with Tuj-1 in immature neurons from a differentiated VZ neurosphere. Hoechst 33342 was used as a nuclear counterstain in C-E. Scalebars 10 μm. DCX, doublecortin; GFAP, glial fibrillary acidic protein; NPC, neural progenitor cells; NSC, neural stem cells; Panx1, pannexin1; SGZ, subgranular zone; VZ, ventricular zone.

We then examined the impact of the Panx1-blocker, probenecid, on the proliferative capacity of VZ neurosphere cultures. Neurospheres were cultured in the absence or presence of 1 mM probenecid from DIV1 onwards. Neurospheres were observed each day by light microscopy and diameter was measured on DIV7 (Figure 5A). Probenecid-treated neurospheres were significantly smaller than controls (41.85 ± 1.649 μm and 93.97 ± 5.089 μm, respective1y, P < 0.0001 for non-parametric t test, N = 12) (Figure 5B).

Figure 5
figure 5

Panx1 regulates primary NSC/NPC proliferation. (A) Outline of neurosphere treatments in vitro. Growth factors (hEGF and bFGF) were added every two DIV beginning at DIV0, while the Panx1 blocker probenecid was added at DIV1 to a concentration of 1 mM. Sphere diameter was measured at DIV7. (B) Brightfield images of vehicle and probenecid treated spheres (i). A significant reduction in neurosphere diameter was observed following probenecid treatment compared to vehicle treatment (41.85 ± 1.649 μm and 93.97 ± 5.089 μm respectively, P < 0.0001 for non-parametric t test, N = 12) (ii). DIV, days in vitro; NPC, neural progenitor cells; NSC, neural stem cells; Panx1, pannexin 1.

Discussion

Here we report the discovery of Panx1 expression in NSC/NPCs both in vivo and in vitro. In vivo, Panx1 expression was primarily found in luminal type B NSCs and in some sub-ependymal NSCs. As described above, electron microscopy has shown that the ependymal layer lining the ventricles is not entirely contiguous and that a large proportion of type B cells make direct contact with the ventricle either via a large luminal surface or a thin cellular process [38]. Panx1 block by probenecid reduced NSC/NPC proliferation, while overexpression of Panx1 increased proliferation. Although the Panx1 blocker probenecid is also known to target organic anion transporters [46, 47], the increase in cell proliferation caused by Panx1 overexpression was almost completely blocked by probenecid suggesting that this is a Panx1-specific effect.

As large pore ion and metabolite-permeable channels, Panx1 make ideal integrators of neurogenic microenvironment signaling in the periventricular region. Accordingly, our results demonstrate a role for Panx1 in the regulation of NSC/NPC proliferation, in part, through mediating the release of ATP (or similar nucleotides like ADP and UTP) that subsequently acts on P2 purinergic receptors [2225]. Here we induced ATP release by stimulating the cells with elevated extracellular potassium (20 mM), and found a significant reduction in this stimulated release upon probenecid treatment. ATP is also basally secreted in bursts from NSC/NPCs. In other cell types, ATP activates Panx1 channels in a P2-receptor-dependent manner [48]. A similar nucleotide (ATP, ADP or UTP)-dependent positive feedback mechanism could account for the increased proliferation observed under Panx1 overexpression, as proliferation is blocked by the P2 receptor blocker PPADS. We did not detect a large increase in basal ATP levels with Panx1 overexpression, but this is not surprising given the high expression of 5′-nucleotidase on the surface of NSC/NPCs [22].

What additional signals might be involved in triggering ATP release from Panx1 in NSC/NPCs? Recently an interaction between Panx1 and the actin cytoskeleton was described [43] in BICR-M1R(k) cells, and Panx1 has previously been shown to be activated by cell membrane stretch [18, 20]. Multiple cytoskeletal rearrangements occur in cell division and might perpetuate Panx1 mediated ATP release and downstream purinergic receptor signaling, resulting in continued proliferation. Interestingly, we detected cleavage fragments of Panx1 consistent with recently reported Panx1 caspase 3 cleavage that results in constitutive channel opening during apoptosis, and release of ‘find me’ nucleotide signals necessary for the recruitment of phagocytic cells [16]. Our knowledge of the role of caspases has recently expanded from apoptosis to include many other non-apoptotic cellular roles, including cell proliferation and differentiation (reviewed in [49]). It is tempting to speculate that constitutive Panx1 activity generated by caspase proteolytic cleavage might also be relevant to, or necessary for, its role in NSC/NPC proliferation. As PPADS did not reduce proliferation to the same extent as probenecid (Figure 2), the possibility exists that other Panx1 mechanisms may be involved in addition to an ATP/nucleotide release. Using unbiased proteomic methods, we are actively pursuing the identification of Panx1 protein interaction partners specific to NSC/NPC to elucidate further the signaling pathway(s) that regulate Panx1 function in NSC/NPCs.

The expression and functional role of Panx2 in NSC/NPCs was recently described [10]. In accordance with previous studies in heterologous expression systems [50], the current study demonstrates Panx1 has a very different subcellular distribution profile than Panx2 in NSC/NPCs. While Panx2 was mainly found in discrete intracellular structures, Panx1 is more widely distributed and also found at the plasma membrane (Figures 134). Also, while Panx2 expression was limited to a small subset of cells, Panx1 appears to be abundantly expressed. Furthermore, recent work suggests that the oligomeric structures and pharmacological sensitivities of Panx1 and Panx2 are fundamentally different [51], suggesting that a physical or functional interaction in NSC/NPCs is unlikely.

During embryonic development, the source of ATP release from VZ radial glia has been reported to be connexin 43 hemichannels [52]. Panx1 channels have only recently come into the picture, having been discovered long after the connexins by homology to the invertebrate innexins [12]. Given the relatively high embryonic expression of Panx1 [15] and the data we present here on postnatal Panx1 expression in postnatal periventricular NSC/NPCs, it would be interesting, in future studies, to examine potential crosstalk or overlap between connexin 43 and Panx1 signaling in NSC/NPCs during embryonic development. For example, perhaps there are subpopulations of NSC/NPCs that express either connexin 43 or Panx1, or if co-expressed, are coupled to or regulated by different signaling paradigms. We do not detect Panx1 in postnatal migrating neuroblasts whereas connexin 43 appears to be expressed in these cells [53], and has been shown to play a major role in newborn cell migration from the periventricular region in the embryonic brain [5456]; this suggests that the roles of Panx1 and connexin 43 are functionally and somewhat physically distinct.

Recent studies highlight the reparative potential of enhancing the proliferation, recruitment and survival of periventricular neuroblasts (reviewed in [5759]). To eventually harness this potential it is important to understand the factors regulating the behavior of these cells, including the role of Panx1. Panx1 ion and metabolite-permeable channels are known to open in several pathological circumstances, including elevated extracellular potassium [28], ischemia [60] and elevated amyloid beta peptide exposure [21], a pathogenic feature of Alzheimer’s disease (AD). NSCs are highly sensitive to changes in their environment, especially injury and neurological diseases (reviewed in [61, 62]). Some of the changes in neurogenesis seen with brain injury, stroke and AD could conceivably involve pathological activation of Panx1 channels in NSC/NPCs. Therefore, understanding the fundamental role of Panx1 channels in NSC/NPCs is of high importance for brain health and disease.

Conclusions

In this study we have established that Panx1 is expressed in NSC/NPCs where it plays an important role in the positive regulation of cell proliferation. As previous work has focused mainly on the role of Panx1 in neuropathology, this is, to our knowledge, the first demonstration of a normal physiological role of Panx1 channels in the central nervous system. After discovering Panx1 expression in postnatal VZ NSC/NPCs, in vivo, we then sought to elucidate its functional role in neuronal development by manipulating Panx1 expression and activity in N2a cells and primary postnatal neurosphere cultures. We demonstrated that Panx1 channels mediated ATP release, which contributes to positive regulation of proliferation through activation of P2 receptors. Moreover, we found that overexpression of Panx1 robustly and significantly increased N2a cell proliferation and that these effects were abrogated by the Panx1 blocker, probenecid. Furthermore, we detected robust Panx1 expression in postnatal VZ neurosphere cultures. As anticipated, blocking Panx1 activity in these primary NSC/NPC cultures significantly reduced cell proliferation. Future studies are now needed to expand our knowledge of the properties of Panx1 channels in NSC/NPCs, including additional possible mechanisms underlying their role in the regulation of proliferation. Understanding the fundamental roles of Panx1 channels in NSC/NPCs is of critical importance given the vital roles that postnatal neurogenesis plays in normal brain health, as well in the progression of neurological diseases and the response to brain injury.

Methods

Animals

C57BL/6 mice were used and all procedures were carried out in agreement with the guidelines of the Canadian Council for Animal Care and the University of Victoria Animal Care Committee.

Cell culture

Primary NSC/NPC cultures were isolated from postnatal day 0 to3 (P0 to P3) C57BL/6 mouse hippocampus and periventricular zone and expanded as neurospheres for seven days in vitro (DIV) as described [9, 43]. For proliferation assays, neurospheres were treated at DIV1 with 1 mM probenecid (Invitrogen), 30 μM pyridoxal phosphate-6-azophenyl-2′,4′-disulfonic acid (PPADS; Sigma, Oakville, Ontario Canada) or equivalent volumes of sterile water. At DIV7, neurospheres were visualized/photographed using brightfield illumination, and sphere diameters were measured in Photoshop CS5 (Adobe). N2a cells were cultured in DMEM/F12 supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin (all obtained from Gibco/Life Technologies, Burlington, Ontario, Canada). Where indicated, N2a cells were transfected using jetPEI reagent (Polyplus transfection/VWR; Edmonton, Alberta, Canada) according to the manufacturer’s protocol, and Panx1 plasmids were a generous gift from Dr. Dale Laird, University of Western Ontario [43, 63]. N2a cells were treated at time 0 with 1 mM probenecid or an equivalent volume of sterile water for proliferation assays, in which cells from a minimum of three wells per condition were counted in quintuplicate at the indicated timepoints.

Reverse transcriptase-polymerase chain reaction (RT-PCR)

Total RNA was isolated and first-strand synthesis and PCR were carried out as described [43] using the following cycling parameters: 94°C for 5 minutes, 35 cycles of 94°C for 30 seconds, 57°C for 50 seconds, and 72°C for 2 minutes, and a final step at 72°C for 7 minutes. Primers were: 5′-CATTGACCCCATGCTACTCC-3′, 5′- TCAGCCACAGAAGTCACAGG-3′ defining a 248 bp Panx1 amplicon, accession# [GenBank: NM_019482.2)] and 5′-TGGTGCTGAGTATGTCGTGGAGT-3′, 5′-AGTCTTCTGAGTGGCAGTGATGG-3′ defining a 292 bp glyceraldehyde-3-phosphate dehydrogenase (GAPDH) amplicon, accession# [GenBank: NM_008084.2].

Western analyses

Western analysis was performed as described [9, 43]. Samples were homogenized in RIPA buffer (10 mM PBS: 150 mM NaCl, 9.1 mM dibasic sodium phosphate, 1.7 mM monobasic sodium phosphate), 1% Nonidet P-40, 0.5% sodium deoxycholate, 0.1% SDS, protease inhibitor cocktail at 1 μL/106 cells (stock: 0.104 mM 4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride, 0.08 mM aprotinin, 4 mM bestatin hydrochloride, 1.4 mM N-(trans-epoxysuccinyl)-L-leucine 4-guanidinobutylamide, 2 mM leupeptin hemisulfate salt, 1.5 mM pepstatin-A; Sigma-Aldrich) for 30 minutes and centrifuged for 20 minutes at 12,000 rpm to remove debris. For all Western blots, SDS-PAGE was performed under reducing conditions (dithiothreitol (DTT) and β-mercaptoethanol) without heating.

ATP release assay

N2a cells were plated at a density of 1.8 × 104 cells/cm2 24 hours prior to ATP assay. Media was removed and cells were washed once with Hank’s balanced salt solution (HBSS; 137.93 mM NaCl, 5.33 mM KCl, 1.26 mM CaCl2, 0.9 mM MgCl2, 5.56 mM D-glucose, 0.441 mM KH2PO4, 4.17 mM NaHCO3, 0.338 mM Na2HPO4) and pre-incubated for 10 minutes in HBSS with or without the addition of 0.5 mM probenecid. Eighty percent of the pre-incubation medium was removed, and replaced with an equal volume of high KCl HBSS (117.93 mM NaCl, 25 mM KCl, 1.26 mM CaCl2, 0.9 mM MgCl2, 5.56 mM D-glucose, 0.441 mM KH2PO4, 4.17 mM NaHCO3, 0.338 mM Na2HPO4) for a final concentration of 20 mM KCl with or without 0.5 mM probenecid, for 10 minutes. Eighty percent of the treatment medium was removed, spun down for 1 minute at 13,500 rpm, and the top portion was analyzed in triplicate for ATP concentration using an ATP determination kit from Molecular Probes/Life Technologies, Burlington, Ontario, Canada). Data are from five independent replicates and were normalized to cell number.

Confocal microscopy

Neurosphere, P15, P30 and P60 mouse brain cryopreservation, serial cryosectioning (10 μm for neurosphere, 20 μm for brain) were performed as described [10, 44, 64]. Antibodies were diluted in 10 mM PBS supplemented with 0.3% Triton-X-100 and 3% BSA. Primary antibodies were: rabbit polyclonal anti-Panx1 Cterm (1:40 Invitrogen), rat monoclonal anti-GFAP (1:40, Invitrogen/Life Technologies, Burlington, Ontario, Canada), mouse monoclonal anti-nestin (1:100, Chemicon/Millipore, Temecula, California, USA), guinea pig polyclonal anti-doublecortin (DCX; 1:800, Chemicon/Millipore), mouse monoclonal anti-Tuj1 class III β-tubulin (1:250, RDI). Secondary antibodies were Cy3-conjugated anti-rabbit IgG (1:600), DyLight488-conjugated anti-rabbit IgG (1:100), Cy3-conjugated anti-guinea-pig IgG (1:800), DyLight488-conjugated anti-mouse IgG (1:600), and DyLight649-conjugated anti-mouse IgG (1:400). Hoechst 33342 (1 μg/mL) was used as a nuclear counterstain. To label N2a cell membranes, cells were treated with 5 μg/mL TRITC-conjugated wheat germ agglutinin (WGA, Molecular Probes/Life Technologies) for 5 minutes at 37°C in HBSS (137 mM NaCl, 5.4 mM KCl, 0.25 mM Na2HPO4, 0.44 mM KH2PO4, 4.2 mM NaHCO3; Gibco/Life Technologies), washed in HBSS, fixed in 3.7% paraformaldehyde for 15 minutes and processed for immunostaining. Confocal immunofluorescence imaging was performed on a Zeiss LSM 700 confocal microscope and images were captured sequentially with ZEN 2009 software on an EC Plan-Neofluar 40x/1.30 oil DIC M27 objective with equal optical slices for multiple fluorophores (approximately 1 Airy Unit) through z-stacks in 0.35 μm optical sections.

Statistical analyses

Significance was determined using Student’s t tests or ANOVA with Tukey multiple comparisons post-hoc test. Variances are reported as standard error of the mean.

Authors’ information

LAS is an Assistant Professor in the Division of Medical Sciences at the University of Victoria.

Abbreviations

AD:

Alzheimer’s disease

ANOVA:

analysis of variance

bFGF:

basic fibroblast growth factor

BSA:

bovine serum albumin

DIV:

days in vitro

DTT:

dithiothreitol

DCX:

doublecortin

DMEM/F12:

Dulbecco’s modified eagle medium/F12

EGFP:

enhanced green fluorescent protein

FBS:

fetal bovine serum

GFAP:

glial fibrillary acidic protein

GAPDH:

glyceraldehyde-3-phosphate dehydrogenase

HBSS:

Hank’s buffered salt solution

HEK293T cells:

human embryonic kidney 293T cells

hEGF:

human epidermal growth factor

NPC:

neural progenitor cell

NSC:

neural stem cell

N2a:

Neuro2a

Panx:

pannexin

PBS:

phosphate buffered saline

P:

postnatal day

PPADS:

pyridoxalphosphate-6-azophenyl-2′,4′-disulfonic acid

RT-PCR:

reverse-transcriptase polymerase chain reaction

SGZ:

subgranular zone

Tuj-1:

class III beta tubulin

UTP:

uridine triphosphate

V:

ventricle

VZ:

ventricular zone

WGA:

wheat germ agglutinin.

References

  1. Ming GL, Song H: Adult neurogenesis in the mammalian brain: significant answers and significant questions. Neuron. 2011, 70: 687-702. 10.1016/j.neuron.2011.05.001.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  2. Swayne LA, Wicki-Stordeur LE: Ion channels in postnatal neurogenesis: potential targets for brain repair. Channels (Austin) 2012, in press

  3. Doetsch F, Caille I, Lim DA, Garcia-Verdugo JM, Alvarez-Buylla A: Subventricular zone astrocytes are neural stem cells in the adult mammalian brain. Cell. 1999, 97: 703-716. 10.1016/S0092-8674(00)80783-7.

    Article  CAS  PubMed  Google Scholar 

  4. Conover JC, Doetsch F, Garcia-Verdugo JM, Gale NW, Yancopoulos GD, Alvarez-Buylla A: Disruption of Eph/ephrin signaling affects migration and proliferation in the adult subventricular zone. Nat Neurosci. 2000, 3: 1091-1097. 10.1038/80606.

    Article  CAS  PubMed  Google Scholar 

  5. Merkle FT, Mirzadeh Z, Alvarez-Buylla A: Mosaic organization of neural stem cells in the adult brain. Science. 2007, 317: 381-384. 10.1126/science.1144914.

    Article  CAS  PubMed  Google Scholar 

  6. Mirzadeh Z, Merkle FT, Soriano-Navarro M, Garcia-Verdugo JM, Alvarez-Buylla A: Neural stem cells confer unique pinwheel architecture to the ventricular surface in neurogenic regions of the adult brain. Cell Stem Cell. 2008, 3: 265-278. 10.1016/j.stem.2008.07.004.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  7. Shen Q, Wang Y, Kokovay E, Lin G, Chuang SM, Goderie SK, Roysam B, Temple S: Adult SVZ stem cells lie in a vascular niche: a quantitative analysis of niche cell-cell interactions. Cell Stem Cell. 2008, 3: 289-300. 10.1016/j.stem.2008.07.026.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  8. Doetsch F, Petreanu L, Caille I, Garcia-Verdugo JM, Alvarez-Buylla A: EGF converts transit-amplifying neurogenic precursors in the adult brain into multipotent stem cells. Neuron. 2002, 36: 1021-1034. 10.1016/S0896-6273(02)01133-9.

    Article  CAS  PubMed  Google Scholar 

  9. Yasuda T, Adams DJ: Physiological roles of ion channels in adult neural stem cells and their progeny. J Neurochem. 2010, 114: 946-959.

    CAS  PubMed  Google Scholar 

  10. Swayne LA, Sorbara CD, Bennett SA: Pannexin 2 is expressed by postnatal hippocampal neural progenitors and modulates neuronal commitment. J Biol Chem. 2010, 285: 24977-24986. 10.1074/jbc.M110.130054.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  11. Zappala A, Cicero D, Serapide MF, Paz C, Catania MV, Falchi M, Parenti R, Panto MR, La Delia F, Cicirata F: Expression of pannexin1 in the CNS of adult mouse: cellular localization and effect of 4-aminopyridine-induced seizures. Neuroscience. 2006, 141: 167-178. 10.1016/j.neuroscience.2006.03.053.

    Article  CAS  PubMed  Google Scholar 

  12. Bruzzone R, Hormuzdi SG, Barbe MT, Herb A, Monyer H: Pannexins, a family of gap junction proteins expressed in brain. Proc Natl Acad Sci U S A. 2003, 100: 13644-13649. 10.1073/pnas.2233464100.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  13. Wang XH, Streeter M, Liu YP, Zhao HB: Identification and characterization of pannexin expression in the mammalian cochlea. J Comp Neurol. 2009, 512: 336-346. 10.1002/cne.21898.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  14. Zoidl G, Petrasch-Parwez E, Ray A, Meier C, Bunse S, Habbes HW, Dahl G, Dermietzel R: Localization of the pannexin1 protein at postsynaptic sites in the cerebral cortex and hippocampus. Neuroscience. 2007, 146: 9-16. 10.1016/j.neuroscience.2007.01.061.

    Article  CAS  PubMed  Google Scholar 

  15. Vogt A, Hormuzdi SG, Monyer H: Pannexin1 and Pannexin2 expression in the developing and mature rat brain. Brain Res Mol Brain Res. 2005, 141: 113-120.

    Article  CAS  PubMed  Google Scholar 

  16. Chekeni FB, Elliott MR, Sandilos JK, Walk SF, Kinchen JM, Lazarowski ER, Armstrong AJ, Penuela S, Laird DW, Salvesen GS, Isakson BE, Bayliss DA, Ravichandran KS: Pannexin 1 channels mediate ‘find-me’ signal release and membrane permeability during apoptosis. Nature. 2010, 467: 863-867. 10.1038/nature09413.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  17. MacVicar BA, Thompson RJ: Non-junction functions of pannexin-1 channels. Trends Neurosci. 2009, 33: 93-102.

    Article  PubMed  Google Scholar 

  18. Bao L, Locovei S, Dahl G: Pannexin membrane channels are mechanosensitive conduits for ATP. FEBS Lett. 2004, 572: 65-68. 10.1016/j.febslet.2004.07.009.

    Article  CAS  PubMed  Google Scholar 

  19. Schenk U, Westendorf AM, Radaelli E, Casati A, Ferro M, Fumagalli M, Verderio C, Buer J, Scanziani E, Grassi F: Purinergic control of T cell activation by ATP released through pannexin-1 hemichannels. Sci Signal. 2008, 1: ra6-10.1126/scisignal.1160583.

    Article  PubMed  Google Scholar 

  20. Seminario-Vidal L, Okada SF, Sesma JI, Kreda SM, van Heusden CA, Zhu Y, Jones LC, O’Neal WK, Penuela S, Laird DW, Boucher RC, Lazarowski ER: Rho signaling regulates pannexin 1-mediated ATP release from airway epithelia. J Biol Chem. 2011, 286: 26277-26286. 10.1074/jbc.M111.260562.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  21. Orellana JA, Shoji KF, Abudara V, Ezan P, Amigou E, Saez PJ, Jiang JX, Naus CC, Saez JC, Giaume C: Amyloid beta-induced death in neurons involves glial and neuronal hemichannels. J Neurosci. 2011, 31: 4962-4977. 10.1523/JNEUROSCI.6417-10.2011.

    Article  CAS  PubMed  Google Scholar 

  22. Mishra SK, Braun N, Shukla V, Fullgrabe M, Schomerus C, Korf HW, Gachet C, Ikehara Y, Sevigny J, Robson SC, Zimmermann H: Extracellular nucleotide signaling in adult neural stem cells: synergism with growth factor-mediated cellular proliferation. Development. 2006, 133: 675-684. 10.1242/dev.02233.

    Article  CAS  PubMed  Google Scholar 

  23. Lin JH, Takano T, Arcuino G, Wang X, Hu F, Darzynkiewicz Z, Nunes M, Goldman SA, Nedergaard M: Purinergic signaling regulates neural progenitor cell expansion and neurogenesis. Dev Biol. 2007, 302: 356-366. 10.1016/j.ydbio.2006.09.017.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  24. Hogg RC, Chipperfield H, Whyte KA, Stafford MR, Hansen MA, Cool SM, Nurcombe V, Adams DJ: Functional maturation of isolated neural progenitor cells from the adult rat hippocampus. Eur J Neurosci. 2004, 19: 2410-2420. 10.1111/j.0953-816X.2004.03346.x.

    Article  PubMed  Google Scholar 

  25. Wu PY, Lin YC, Chang CL, Lu HT, Chin CH, Hsu TT, Chu D, Sun SH: Functional decreases in P2X7 receptors are associated with retinoic acid-induced neuronal differentiation of Neuro-2a neuroblastoma cells. Cell Signal. 2009, 21: 881-891. 10.1016/j.cellsig.2009.01.036.

    Article  CAS  PubMed  Google Scholar 

  26. Huang Y, Grinspan JB, Abrams CK, Scherer SS: Pannexin1 is expressed by neurons and glia but does not form functional gap junctions. Glia. 2007, 55: 46-56. 10.1002/glia.20435.

    Article  PubMed  Google Scholar 

  27. Shea TB, Fischer I, Sapirstein VS: Effect of retinoic acid on growth and morphological differentiation of mouse NB2a neuroblastoma cells in culture. Brain Res. 1985, 353: 307-314.

    Article  CAS  PubMed  Google Scholar 

  28. Silverman WR, de Rivero Vaccari JP, Locovei S, Qiu F, Carlsson SK, Scemes E, Keane RW, Dahl G: The pannexin 1 channel activates the inflammasome in neurons and astrocytes. J Biol Chem. 2009, 284: 18143-18151. 10.1074/jbc.M109.004804.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  29. Santiago MF, Veliskova J, Patel NK, Lutz SE, Caille D, Charollais A, Meda P, Scemes E: Targeting pannexin1 improves seizure outcome. PLoS One. 2011, 6: e25178-10.1371/journal.pone.0025178.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  30. Wolfgang W: Potassium homeostasis in the brain at the organ and cell level. Advances in Molecular and Cell Biology, Volume 31. Edited by: Leif H. 2003, Elsevier, , 595-609.

    Google Scholar 

  31. Silverman W, Locovei S, Dahl G: Probenecid, a gout remedy, inhibits pannexin 1 channels. Am J Physiol Cell Physiol. 2008, 295: C761-C767. 10.1152/ajpcell.00227.2008.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  32. Liu T, Sun L, Xiong Y, Shang S, Guo N, Teng S, Wang Y, Liu B, Wang C, Wang L, Zheng L, Zhang CX, Han W, Zhou Z: Calcium triggers exocytosis from two types of organelles in a single astrocyte. J neurosci. 2011, 31: 10593-10601. 10.1523/JNEUROSCI.6401-10.2011.

    Article  CAS  PubMed  Google Scholar 

  33. Stout CE, Costantin JL, Naus CC, Charles AC: Intercellular calcium signaling in astrocytes via ATP release through connexin hemichannels. J Biol Chem. 2002, 277: 10482-10488. 10.1074/jbc.M109902200.

    Article  CAS  PubMed  Google Scholar 

  34. Iglesias R, Dahl G, Qiu F, Spray DC, Scemes E: Pannexin 1: the molecular substrate of astrocyte “hemichannels”. J Neurosci. 2009, 29: 7092-7097. 10.1523/JNEUROSCI.6062-08.2009.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  35. Lambrecht G, Friebe T, Grimm U, Windscheif U, Bungardt E, Hildebrandt C, Baumert HG, Spatz-Kumbel G, Mutschler E: PPADS, a novel functionally selective antagonist of P2 purinoceptor-mediated responses. Eur J Pharmacol. 1992, 217: 217-219. 10.1016/0014-2999(92)90877-7.

    Article  CAS  PubMed  Google Scholar 

  36. Khakh BS, Burnstock G, Kennedy C, King BF, North RA, Seguela P, Voigt M, Humphrey PP: International union of pharmacology. XXIV. Current status of the nomenclature and properties of P2X receptors and their subunits. Pharmacol Rev. 2001, 53: 107-118.

    CAS  PubMed  Google Scholar 

  37. Khakh BS: Molecular physiology of P2X receptors and ATP signalling at synapses. Nat Rev Neurosci. 2001, 2: 165-174.

    Article  CAS  PubMed  Google Scholar 

  38. Troadec JD, Thirion S, Petturiti D, Bohn MT, Poujeol P: ATP acting on P2Y receptors triggers calcium mobilization in primary cultures of rat neurohypophysial astrocytes (pituicytes). Pflugers Arch. 1999, 437: 745-753. 10.1007/s004240050841.

    Article  CAS  PubMed  Google Scholar 

  39. North RA: Molecular physiology of P2X receptors. Physiol Rev. 2002, 82: 1013-1067.

    Article  CAS  PubMed  Google Scholar 

  40. Communi D, Motte S, Boeynaems JM, Pirotton S: Pharmacological characterization of the human P2Y4 receptor. Eur J Pharmacol. 1996, 317: 383-389. 10.1016/S0014-2999(96)00740-6.

    Article  CAS  PubMed  Google Scholar 

  41. Communi D, Robaye B, Boeynaems JM: Pharmacological characterization of the human P2Y11 receptor. Br J Pharmacol. 1999, 128: 1199-1206. 10.1038/sj.bjp.0702909.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  42. Boyer JL, Zohn IE, Jacobson KA, Harden TK: Differential effects of P2-purinoceptor antagonists on phospholipase C- and adenylyl cyclase-coupled P2Y-purinoceptors. Br J Pharmacol. 1994, 113: 614-620.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  43. Bhalla-Gehi R, Penuela S, Churko JM, Shao Q, Laird DW: Pannexin1 and pannexin3 delivery, cell surface dynamics, and cytoskeletal interactions. J Biol Chem. 2010, 285: 9147-9160. 10.1074/jbc.M109.082008.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  44. Imbeault S, Gauvin LG, Toeg HD, Pettit A, Sorbara CD, Migahed L, DesRoches R, Menzies AS, Nishii K, Paul DL, Simon AM, Bennett SA: The extracellular matrix controls gap junction protein expression and function in postnatal hippocampal neural progenitor cells. BMC Neurosci. 2009, 10: 13-10.1186/1471-2202-10-13.

    Article  PubMed Central  PubMed  Google Scholar 

  45. Ray A, Zoidl G, Weickert S, Wahle P, Dermietzel R: Site-specific and developmental expression of pannexin1 in the mouse nervous system. Eur J Neurosci. 2005, 21: 3277-3290. 10.1111/j.1460-9568.2005.04139.x.

    Article  PubMed  Google Scholar 

  46. Enomoto A, Kimura H, Chairoungdua A, Shigeta Y, Jutabha P, Cha SH, Hosoyamada M, Takeda M, Sekine T, Igarashi T, Matsuo H, Kikuchi Y, Oda T, Ichida K, Hosoya T, Shimokata K, Niwa T, Kanai Y, Endou H: Molecular identification of a renal urate anion exchanger that regulates blood urate levels. Nature. 2002, 417: 447-452.

    CAS  PubMed  Google Scholar 

  47. May K, Minarikova V, Linnemann K, Zygmunt M, Kroemer HK, Fusch C, Siegmund W: Role of the multidrug transporter proteins ABCB1 and ABCC2 in the diaplacental transport of talinolol in the term human placenta. Drug Metab Dispos. 2008, 36: 740-744. 10.1124/dmd.107.019448.

    Article  CAS  PubMed  Google Scholar 

  48. Locovei S, Wang J, Dahl G: Activation of pannexin 1 channels by ATP through P2Y receptors and by cytoplasmic calcium. FEBS Lett. 2006, 580: 239-244. 10.1016/j.febslet.2005.12.004.

    Article  CAS  PubMed  Google Scholar 

  49. Lamkanfi M, Festjens N, Declercq W, Vanden Berghe T, Vandenabeele P: Caspases in cell survival, proliferation and differentiation. Cell Death Differ. 2007, 14: 44-55. 10.1038/sj.cdd.4402047.

    Article  CAS  PubMed  Google Scholar 

  50. Penuela S, Bhalla R, Nag K, Laird DW: Glycosylation regulates pannexin intermixing and cellular localization. Mol Biol Cell. 2009, 20: 4313-4323. 10.1091/mbc.E09-01-0067.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  51. Ambrosi C, Gassmann O, Pranskevich JN, Boassa D, Smock A, Wang J, Dahl G, Steinem C, Sosinsky GE: Pannexin1 and Pannexin2 channels show quaternary similarities to connexons and different oligomerization numbers from each other. J Biol Chem. 2010, 285: 24420-24431. 10.1074/jbc.M110.115444.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  52. Weissman TA, Riquelme PA, Ivic L, Flint AC, Kriegstein AR: Calcium waves propagate through radial glial cells and modulate proliferation in the developing neocortex. Neuron. 2004, 43: 647-661. 10.1016/j.neuron.2004.08.015.

    Article  CAS  PubMed  Google Scholar 

  53. Liu X, Bolteus AJ, Balkin DM, Henschel O, Bordey A: GFAP-expressing cells in the postnatal subventricular zone display a unique glial phenotype intermediate between radial glia and astrocytes. Glia. 2006, 54: 394-410. 10.1002/glia.20392.

    Article  PubMed  Google Scholar 

  54. Elias LA, Wang DD, Kriegstein AR: Gap junction adhesion is necessary for radial migration in the neocortex. Nature. 2007, 448: 901-907. 10.1038/nature06063.

    Article  CAS  PubMed  Google Scholar 

  55. Ewart JL, Cohen MF, Meyer RA, Huang GY, Wessels A, Gourdie RG, Chin AJ, Park SM, Lazatin BO, Villabon S, Lo CW: Heart and neural tube defects in transgenic mice overexpressing the Cx43 gap junction gene. Development. 1997, 124: 1281-1292.

    CAS  PubMed  Google Scholar 

  56. Cina C, Maass K, Theis M, Willecke K, Bechberger JF, Naus CC: Involvement of the cytoplasmic C-terminal domain of connexin43 in neuronal migration. J Neurosci. 2009, 29: 2009-2021.

    Article  CAS  PubMed  Google Scholar 

  57. Liu YP, Lang BT, Baskaya MK, Dempsey RJ, Vemuganti R: The potential of neural stem cells to repair stroke-induced brain damage. Acta Neuropathol. 2009, 117: 469-480. 10.1007/s00401-009-0516-1.

    Article  PubMed  Google Scholar 

  58. Leker RR, Lasri V, Chernoguz D: Growth factors improve neurogenesis and outcome after focal cerebral ischemia. J Neural Transm. 2009, 116: 1397-1402. 10.1007/s00702-009-0329-3.

    Article  PubMed  Google Scholar 

  59. Burns TC, Verfaillie CM, Low WC: Stem cells for ischemic brain injury: a critical review. J Comp Neurol. 2009, 515: 125-144. 10.1002/cne.22038.

    Article  PubMed Central  PubMed  Google Scholar 

  60. Thompson RJ, Zhou N, MacVicar BA: Ischemia Opens Neuronal Gap Junction Hemichannels. Science. 2006, 312: 924-927. 10.1126/science.1126241.

    Article  CAS  PubMed  Google Scholar 

  61. Lazarov O, Marr RA: Neurogenesis and Alzheimer’s disease: at the crossroads. Exp Neurol. 2010, 223: 267-281. 10.1016/j.expneurol.2009.08.009.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  62. Lazarov O, Mattson MP, Peterson DA, Pimplikar SW, van Praag H: When neurogenesis encounters aging and disease. Trends Neurosci. 2010, 33: 569-579. 10.1016/j.tins.2010.09.003.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  63. Penuela S, Bhalla R, Gong X-Q, Cowan KN, Celetti SJ, Cowan BJ, Bai D, Shao Q, Laird DW: Pannexin 1 and pannexin 3 are glycoproteins that exhibit many distinct characteristics from the connexin family of gap junction proteins. J Cell Sci. 2007, 120: 3772-3783. 10.1242/jcs.009514.

    Article  CAS  PubMed  Google Scholar 

  64. Melanson-Drapeau L, Beyko S, Dave S, Hebb ALO, Franks DJ, Sellitto C, Paul DL, Bennett SAL: Oligodendrocyte Progenitor Enrichment in the Connexin32 Null-Mutant Mouse. J Neurosci. 2003, 23: 1759-1768.

    CAS  PubMed  Google Scholar 

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Acknowledgements

This work was supported by a Natural Sciences and Engineering Research Council of Canada Discovery Grant, and a University of Victoria laboratory start-up grant awarded to LAS. We thank Jen Graham for assistance with mouse perfusions and decapitations, and we thank Dr. Robert Burke and the UVIC Department of Biochemistry and Microbiology for confocal microscope access.

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Correspondence to Leigh Anne Swayne.

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The authors declare that they have no competing interests.

Authors’ contributions

LWS and LAS planned the experiments, prepared the figures and wrote and revised the manuscript. LWS performed all tissue culture and transfections associated with N2a cells and primary neurospheres. ADD, JMS and RMS also assisted in planning selected experiments. LWS and ADD performed cell growth/proliferation assays and ATP assays. RMS performed RT-PCR and cryosectioning of neurosphere and brains. LWS and JMS performed confocal immunofluorescence microscopy experiments and western blotting. All authors read and approved the final manuscript.

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Wicki-Stordeur, L.E., Dzugalo, A.D., Swansburg, R.M. et al. Pannexin 1 regulates postnatal neural stem and progenitor cell proliferation. Neural Dev 7, 11 (2012). https://doi.org/10.1186/1749-8104-7-11

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