Figure 3.

Expression of MEINOX genes in ventral midbrain. In situ hybridization using digoxigenin-labeled riboprobes against (A) TH, (B) Meis1, (C)Meis2 and (D) Meis3. Immunohistochemistry using antibodies against TH (E, G) and Prep1 (F, H). (A-D) None of the Meis family members is expressed by E15 mesDA neurons. (E,F) TH (E) and Prep1 (F) are co-expressed in mesDA neurons. (G-I) Magnification of dashed box, showing Prep1-positive nuclei in TH-positive cell body. Dorsal to the top. Scale bars, A-D = 200 μm, E, F = 250 μm, G-H = 50 μm. mesDA: mesencephalic dopaminergic.