Figure 6.

Development of central complex innervation defects in apoptosis-blocked dorsomedial (DM) lineage neurons. (A-D) Confocal images of single sections at the level of (A, B) the ellipsoid body and (C, D) the fan-shaped body in wild-type pupal brains at 24 hours after puparium formation (APF). Neuropile labeled with (A, C) nc82 and (B, D) neuronal innervation from DM lineages labeled with mCD8::GFP. (E-H) Confocal images of single sections at the level of (E, F) the ellipsoid body and (G, H) the fan-shaped body in the pupal brains of DM-specific apoptosis-blocked flies at 24 hours APF. (E, G) Neuropile labeled with nc82 and (F, H) neuronal innervation labeled with mCD8::GFP. (F) Perturbations in the innervation of the developing EB are indicated by arrows. (I-L) Confocal images of single sections at the level of (I, J) the ellipsoid body and (K, L) the fan-shaped body and noduli in pupal brains of wild-type at 48 hours APF. Neuropile labeled with (I, K) nc82 and (J, L) neuronal innervation labeled with mCD8::GFP. (M-P) Confocal images of single sections at the level of (M, N) the ellipsoid body and (O, P) the fan-shaped body and noduli in pupal brains of DM-specific apoptosis-blocked flies at 48 hours APF. Neuropile labeled with (M, O) nc82 and (N, P) neuronal innervation labeled with mCD8::GFP. Misinnervation and misarrangement in the developing central complex are indicated by (N, P) arrows and arrowheads (see text for details). Scale bar = 20 μm Genotypes: (A-D, I-L) UAS-flp; erm-Gal4^R09D11 act>CD2>Gal4 UAS-mCD8::GFP (E-H, M-P) UAS-flp; UAS-p35; erm-Gal4^R09D11 act>CD2>Gal4 UAS-mCD8::GFP.