Preliminary experiments to decide landmarks for DiI application to the VN. (A) Schematic diagram showing a flat-mounted hindbrain with the ventricular surface facing up. DiI application was at a site close to the VIIIth cranial nerve root and along the rhombomere 4/5 border (indicated by the asterisk). (B) Brightfield image of a flat-mounted hindbrain (E13.5) after DiI/DMSO was injected at a site (arrowhead) close to the VIIIth cranial nerve root (VIII). (C) Transverse section of the hindbrain at the level of the VIIIth cranial nerve root (VIII) after DiI crystal was applied at a site (arrowhead) approximately 75 μm from the floor of the fourth ventricle and in the alar plate. Abbreviations: 4th, fourth ventricle; ap, alar plate; bp, basal plate; cp, cerebellar primordium; V, Vth cranial nerve root; VIII, VIIIth cranial nerve root; r2 to r7, rhombomeres 2 to 7.
Kwok et al. Neural Development 2012 7:6 doi:10.1186/1749-8104-7-6