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The neurotrophin receptor p75 regulates gustatory axon branching and promotes innervation of the tongue during development

Neural Development volume 9, Article number: 15 (2014) Cite this article

Abstract

Background

Brain-derived neurotrophic factor (BDNF) and neurotrophin-4 (NT4) regulate the survival of gustatory neurons, axon growth and branching, and innervation of taste buds during development. These actions are largely, but not completely, mediated through the tyrosine kinase receptor, TrkB. Here, we investigated the role of p75, the other major receptor for BDNF and NT4, in the development of the taste system.

Results

We found that p75−/−mice showed delayed axon outgrowth and reduced branching of gustatory axons at embryonic day (E)13.5. From E14.5 to E18.5, gustatory neurons innervated fewer papillae and completely failed to innervate the mid-region of the tongue in p75−/−mice. These early effects of the p75 mutation on gustatory axons preceded the loss of geniculate ganglion neurons starting at E14.5 and also contributed to a loss of taste buds at and after birth. Because knockouts for the TrkB receptor (TrkB−/−) do not lose as many taste buds as hybrid knockouts for its two ligands (BDNF and NT4), we asked if p75 maintains those additional taste buds in the absence of TrkB. It does not; hybrid TrkB−/−/p75−/−mice had more taste buds than TrkB−/−mice; these additional taste buds were not due to an increase in neurons or innervation.

Conclusions

p75 regulates gustatory neuron axon branching and tongue innervation patterns during taste system development. This function is likely accomplished independently of BDNF, NT4, and TrkB. In addition, p75 does not support the remaining neurons or taste buds in TrkB−/−mice.

Background

During development, axons from gustatory neurons of the geniculate ganglion grow toward and innervate specific regions of the tongue. These axons follow defined pathways, suggesting that a series of molecular cues in the environment regulate their guidance [1]. Upon arrival in the tongue, these gustatory fibers innervate specialized structures (for example, fungiform placodes) but not the surrounding epithelia. The fibers defasiculate upon entering the epithelium thereby forming a neural bud [2]. In addition to regulating the location of innervation within the tongue, molecular factors such as neurotrophins also control the number of neurons that innervate each taste bud [3].

Brain-derived neurotrophic factor (BDNF), a neurotrophin produced in developing taste papillae, regulates the survival of gustatory neurons [4, 5]. Acting as a chemoattractant [6], BDNF also guides gustatory axons to their final targets [7–9]. Neurotrophin-4 (NT4) also regulates the survival of gustatory neurons, albeit earlier in development than BDNF [10, 11], but has no role in axon guidance [8]. Therefore, these two neurotrophins control different aspects of taste system development. These two factors function differently primarily because of their locations and timing of expression and not due to differential signaling [12, 13]. They accomplish their diverse functions by acting through the same receptors - the tyrosine kinase receptor TrkB and the pan-neurotrophic receptor p75 [14–16]. Recently, we found that neurotrophins guide a small number of TrkB-negative gustatory neurons to fungiform placodes [17]. As p75 also binds BDNF and NT4, it is possible that this receptor could mediate BDNF and NT4 function independently of TrkB. Observations that adult p75 mutant mice have fewer taste buds and geniculate ganglion neurons [18] suggest that p75 regulates the development of the taste system. However, the specific role of p75 in taste system development is difficult to predict, as p75 is known to interact with several molecular factors in a context- or system-specific manner [19–26].

The goal of the present study was to determine the role of p75 in taste system development. In particular, we examined whether p75 is required for geniculate ganglion axon guidance and neuron survival as well as the maintenance of taste buds during embryonic development by comparing tongue innervation patterns and numbers of geniculate ganglion neurons and taste buds between wild-type and mutant (p75−/−) mice. We found that p75 regulates geniculate ganglion axon branching and promotes innervation of the tongue but does not play a prominent role in gustatory neuron survival. Furthermore, p75 does not appear to mediate BDNF or NT4 function.

Results

Embryonic p75−/−mice show reduced geniculate ganglion axon branching and a lack of innervation in the mid-region of the tongue

We previously found that BDNF and NT4 guide a small number of neurons to fungiform placodes independently of TrkB during development [17], leading us to speculate that the p75 receptor may be important for the TrkB-independent gustatory axon guidance. To test this possibility, we labeled geniculate ganglion neurons with DiI and examined innervation of the tongue in wild-type and p75−/−mouse embryos. In wild-type mice on embryonic day (E)13.5, chorda tympani fiber bundles branched from the main nerve bundle toward the surface of the lateral parts of the tongue but did not yet reach the dorsal tongue surface (Figure 1A,C). Even at this early stage, p75−/−mice showed less axon branching than wild-type mice (Figure 1B,D). In wild-type mice on E14.5, fiber bundle branches reached the tongue surface across its entire medial-to-lateral extent, where they formed bulb-like terminations called ‘neural buds’ [2] (Figure 2A). By contrast, p75−/−mice showed less branching and fewer neural buds at the dorsal surface of the tongue, particularly in the mid-region, with only a few neural buds present at the tip and posterior regions of the tongue (Figure 2B-C). Because innervation of the tongue proceeds in a caudal-to-rostral direction during development [27], the presence of neural buds at the tongue tip suggests that axonal growth and targeting is not merely delayed but that branching is also reduced in p75−/−mice.

Figure 1
figure 1

p75−/−mice show reduced gustatory axon branching and innervation of the tongue mid-region on embryonic day 13.5. DiI-labeled geniculate ganglion axons in tongues from a wild-type (A,C) and p75−/−(B,D) mouse. The dorsal view (A,B) shows reduced branching and disrupted rostral/medial progression in a p75−/−mouse. The side view (C,D) more clearly shows the reduction in branching. Scale bar = 250 μm.

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Figure 2
figure 2

p75−/−mice show reduced branching and mid-region tongue innervation on embryonic days 14.5 and 16.5. DiI-labeled chorda tympani axons in wild-type (A,D,F) and p75−/−mice (B,C,E,G) on embryonic day (E) 14.5 (A,B,C) and E16.5 (D,E,F,G). In p75−/−mice, chorda tympani branching was reduced, and innervation often did not reach the tongue surface as neural buds. Nerve branches were particularly absent from the tongue mid-region (B,C,E). By E16.5, nerve fibers reached the tongue tip in p75−/−mice, but branching and neural bud number was reduced (F,G). Scale bar = 250 μm.

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To determine if this reduced innervation in p75−/−mice continues into development, we examined chorda tympani branching in wild-type and p75−/−mice on E16.5 and E18.5. Among wild-type mice, the pattern of axonal branching on E16.5 was similar to that on E14.5 (Figure 2D). p75−/−mice, however, showed an even more obvious disruption in branching, with most branches not entering the mid-region of the tongue (Figure 2E). At the tongue tip, the general pattern of branching was similar between wild-type and p75−/−mice, but the number of neural buds was noticeably reduced in p75−/−mice (Figure 2F,G). On E18.5, there was still an obvious deficit in innervation of the tongue mid-region in p75−/−mice compared with wild-type mice (Figure 3A-F). By sectioning the tongue and examining innervation of its dorsal surface, we confirmed that branches reached the epithelial surface in p75−/−mice but that innervation did not extend as far caudally as in wild-type mice (Figure 3G,H). Therefore, p75 is required for the development of normal chorda tympani branching and innervation of the mid-region of the tongue.

Figure 3
figure 3

p75−/−mice show reduced branching on embryonic day 18.5. DiI-labeled chorda tympani axons in wild-type (A,C,E) and p75−/−(B,D,F) mice. As with the other ages, innervation was particularly sparse in the tongue mid-region of the p75−/−tongue (D) compared to wild-type tongue (C). Sagittal sections near the tongue mid-line show that innervation reached the epithelial surface in both genotypes (G,H), although p75−/−mice had less innervation caudal to the tongue tip. Scale bar = 500 μm for A, B, C, D, E, and F; scale bar = 200 μm for G and H.

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Embryonic p75−/−mice lose geniculate ganglion neurons beginning on embryonic day 14.5

Mice lacking the p75 receptor lose about 25% of geniculate ganglion neurons by adulthood [18], suggesting that our observed reduction in branching in p75−/−mice could be due to a loss of neurons. Geniculate ganglion neuron survival depends on both BDNF and NT4, with a loss of neurons starting at E11.5 in Ntf4−/−mice and at E13.5 in Bdnf−/−mice [5, 11] and a major loss of neurons by E13.5 in TrkB−/−and Bdnf−/−/Ntf4−/−mice [15, 17]. We reasoned that if the p75 receptor mediates geniculate ganglion neuron survival via BDNF or NT4 binding, we should see neuron loss in p75−/−mice by E13.5. To test this hypothesis, we examined geniculate ganglion neuron number in wild-type and p75−/−mice at E13.5 using anti-β-III tubulin antibody (TUJ-1). Geniculate ganglion neurons were easily identified by their clear nuclei and dark cytoplasm (Figure 4A,B). We found no significant neuron loss in p75−/−mice compared with wild-type mice at E13.5 (Figure 5). Therefore, the reduced branching in E13.5 p75−/−mice is not due to a loss of neurons, and p75 does not appear to act through BDNF and NT4 binding to support gustatory neuron survival during early development.

Figure 4
figure 4

p75−/−mice show less of an increase in geniculate ganglion size with embryonic age. Anti-β-III tubulin antibody labeling of the geniculate ganglion (arrows) in wild-type (A,C,E,G) and p75−/−(B,D,F,H) mice. Beginning on embryonic day (E) 14.5 the ganglion appears to be slightly reduced in size in p75−/−mice compared with wild-type mice. Scale bar = 100 μm.

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Figure 5
figure 5

p75−/−mice lose geniculate ganglion neurons starting on embryonic day 14.5. Compared to wild-type mice, the geniculate ganglion of p75−/−mice contains similar numbers of neurons on embryonic day (E)13.5 but fewer neurons on E14.5 (22% decrease), E16.5 (42% decrease), and E18.5 (36% decrease). *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001.

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We next examined mice at later embryonic ages (E14.5, E16.5, and E18.5; Figure 4C-H) to determine when geniculate ganglion neuron loss begins in p75−/−mice. At all three ages, neuron number was significantly lower in p75−/−mice than in wild-type mice (P ≤ 0.05 (E14.5), P ≤ 0.01 (E16.5), P ≤ 0.001 (E18.5); Figure 5), indicating that p75−/−mice start to lose geniculate ganglion neurons at E14.5 and this loss continues throughout embryonic development. Because E14.5 is the age that gustatory nerves start to innervate peripheral targets [8], and neuron number is controlled by the amount of trophic support from these peripheral targets, this loss of neurons may be due to disrupted innervation of targets.

p75−/−mice lack taste buds and taste bud innervation at birth

Neurotrophins are required for the normal development of taste buds by birth [28–30] and p75 is important for maintaining a normal number of taste buds in adulthood [18]. Given the disrupted innervation pattern observed in p75−/−mouse embryos, we hypothesized that they would show a lack of taste buds at birth. We used anti-cytokeratin-8 and anti-P2X3 to label taste buds and their innervation in wild-type and p75−/−mice at birth. We found that p75−/−mice had significantly fewer taste buds than wild-type mice (P < 0.001; Figure 6D). Whereas all taste buds were innervated in wild-type mice, only 80% of taste buds were innervated in p75−/−mice (Figure 6A-C). This finding is consistent with our earlier observation that embryonic p75−/−mice had un-innervated regions of the tongue, particularly the mid-region. When we measured the volume of un-innervated (Figure 6B) and innervated (Figure 6C) taste buds in p75−/−mice, we found that innervated taste buds were significantly larger than un-innervated taste buds (Figure 6E, P ≤ 0.01). Therefore, p75 receptor mutation disrupted innervation of the tongue during development, reducing both the number and volume of taste buds.

Figure 6
figure 6

p75−/−mice have fewer taste buds, and some of their existing taste buds are un-innervated. (A,B,C) Cytokeratin 8-labeled taste buds (green), P2X3-labeled gustatory nerve fibers (red) and cell nuclei (DAPI, blue) can be seen in the lingual epithelium from a wild-type (A) and p75−/−(B,C) mouse. (D) p75−/−mice had fewer taste buds than wild-type mice. (E) p75−/−mice had similarly sized innervated taste buds but smaller un-innervated taste buds compared with wild-type mice. Scale bar = 10 μm for A-C. **P ≤ 0.01.

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It is well established that innervation is required to support the existence of taste buds during postnatal development [31, 32], suggesting that un-innervated taste buds could be lost at later ages. Therefore, we quantified taste bud number in wild-type and p75−/−mice at postnatal day (P)10. Whereas wild-type mice had 116 ± 7 taste buds, p75−/−mice had only 75 ± 2 taste buds. However, no additional taste buds were lost in p75−/−mice at P10 (35%) compared with birth (42%), indicating that un-innervated taste buds may continue to be supported postnatally.

p75 is not responsible for supporting the existence of taste buds in TrkB−/−mice

We previously showed that TrkB−/−mice possess more taste buds than hybrid Bdnf−/−/Nft4−/−mice [17], perhaps because BDNF or NT4 function through other types of receptor in the absence of TrkB. One likely possibility is the p75 receptor, as we showed earlier p75 is required for normal innervation of the tongue surface. If BDNF and NT4 function via p75 to support the additional taste buds in TrkB−/−mice, the number of taste buds should be reduced in hybrid TrkB−/−/p75−/−mice. To test this possibility, we quantified taste buds in TrkB−/−/p75−/−, TrkB−/−, p75−/−, and wild-type mice at E13.5. Surprisingly, we observed an increase in taste bud number in TrkB−/−/p75−/−mice (53 ± 11) compared with TrkB−/−mice (31 ± 8; P < 0.01). The number of taste buds in TrkB−/−/p75−/−mice was similar to that in p75−/−mice (60 ± 9) but still significantly less than in wild-type mice (103 ± 14; P <0.001). Therefore, p75 does not appear to support the remaining taste buds in TrkB−/−mice.

Interestingly, in other systems, p75 causes the death of some neurons in the absence of Trk receptors [33, 34]. Therefore, we speculated that this might also be true for gustatory neurons, with rescued gustatory neurons supporting the additional taste buds in TrkB−/−/p75−/−mice. To test this idea, we quantified geniculate ganglion neuron number in TrkB−/−/p75−/−mice on E13.5, at which time a deficit in neuron number is observed in TrkB−/−mice. We found that TrkB−/−/p75−/−mice also lacked geniculate ganglion neurons at E13.5, with no significant difference in the number of remaining neurons between TrkB−/− (97 ± 18) and TrkB−/−/p75−/−mice (107 ± 10). Therefore, the deletion of p75 does not appear to increase the number of taste buds in TrkB−/−mice by rescuing neurons, which is consistent with our observation that the additional taste buds in TrkB−/−/p75−/−mice did not appear to be innervated (Figure 7A-F). These results indicate that p75 may promote apoptosis in the taste bud in the absence of innervation.

Figure 7
figure 7

Hybrid TrkB−/−/ p75−/−mice have many un-innervated taste buds at birth. Keratin 8-labeled (green) and P2X3-labeled (red) tongue sections from (A) a p75−/−and (B) a TrkB−/−/p75−/−mouse. Whereas the p75−/−mouse had both innervated (D) and un-innervated (C) taste buds, the TrkB−/−/p75−/−mouse had mostly un-innervated taste buds (E,F). Scale bar = 100 μm for A and B; scale bar = 10 μm for C-F.

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Discussion

Our goal was to determine the role of the neurotrophin receptor p75 in taste system development. In particular, because we previously found that a small subpopulation of TrkB-negative gustatory axons require BDNF to navigate to taste buds through an unknown receptor [17], we tested whether p75 could be this receptor and regulate axon guidance and innervation of targets in a manner similar to BDNF. We found that deletion of p75 disrupted innervation of the tongue surface during development. Specifically, peripheral axons branched less and did not innervate the mid-region of the tongue in embryonic p75−/−mice. As this pattern of innervation was unlike that previously observed in BDNF mutant mice [8], p75 probably does not mediate the role of BDNF in axon guidance. We also found that geniculate ganglion neurons were lost after alterations in axonal branching in p75-/- mice, suggesting that disrupted innervation influences neuron survival in p75−/−mice, and not that neuron death disrupts innervation patterns. The reduced innervation to the tongue surface resulted in taste bud loss, with only 80% of remaining taste buds being innervated in p75−/−mice.

In p75−/−mouse embryos, the chorda tympani nerve branched less and failed to innervate the mid-region of the tongue. Because innervation progresses in a caudal/lateral-to-medial/rostral direction as nerve fibers grow into the tongue [2, 35–37], this lack of innervation could be due to delayed or inhibited nerve outgrowth. This possibility is consistent with the finding that blocking p75 function in vitro reduces BDNF-stimulated outgrowth of geniculate ganglion neurons [38]. In the whisker pad, the absence of p75 delays trigeminal innervation, decreases axon branching, and is associated with delayed glial development [39]. Unlike trigeminal axons, however, which eventually innervate their targets, p75−/−mice show a consistent deficit in innervation of the tongue through E18.5, leading to a permanent reduction in the number of taste buds [18].

At all ages observed, most gustatory nerve endings were missing in the middle and caudal areas of the tongue in p75−/−mice. This pattern of innervation is different from that of Bdnf−/−mice, in which branching is increased and fibers extend to all regions of the tongue but fail to innervate fungiform papillae [8]. Thus, although p75 is a receptor for BDNF, it does not appear to mediate the role of BDNF in axon guidance, although it could partially mediate the role of BDNF in axon outgrowth [38]. Rather, it is more likely that p75 functions independently of TrkB ligands and instead interacts with a different factor to influence axon guidance. p75 is known to interact with several different guidance molecules, including ephrins [40]. Also, p75 modulates activity of the chemorepellent semaphorin3A (Sema3A). Dorsal root ganglion neurons in p75−/−mice are hypersensitive to Sema3A [41], and Sema3A acts as a chemorepellent against geniculate ganglion axons in vitro[37], with its repellent effect being neurotrophin-dependent [42]. Sema3A is expressed in the tongue epithelium early in development, and its expression slowly decreases in a lateral-to-medial direction as the tongue becomes innervated [43], thereby preventing premature innervation. Furthermore, Sema3A mutant mice show increased innervation of the tongue mid-line [37]. Thus, our finding of decreased innervation of the mid-region of the tongue in p75−/−mice is consistent with a hypersensitivity to Sema3A. In general, however, interactions of the p75 receptor with many different signaling factors could be important for axon guidance and targeting.

We found that p75−/−mice lose geniculate neurons by E14.5 and that this loss continues through E18.5. Because this loss occurs after neurons become dependent on BDNF and NT4 [5, 11, 44] it is not likely that p75 functions by mediating the effects of neurotrophins on neuron survival, at least not initially. Normally, gustatory nerves reach the tongue epithelium and innervate fungiform placodes by E14.5 [2, 8]. In p75−/−mouse embryos, fewer fungiform papillae were innervated, indicating that neurons failed to reach their targets. Therefore, these neurons could die starting at E14.5 due to a lack of survival factors received from the targets. Consistent with this idea, we observed only a 30% loss in geniculate ganglion neuron number at E14.5, and no neuron loss at E13.5 even though axon branching was already disrupted. Given that alterations in innervation patterns were more severe and occurred earlier than neuron loss, it is more likely that disrupted innervation caused neuron loss rather than vice versa. Neuron loss in other sensory ganglia could also be due to failed targeting [39, 45], which could explain why sensory neurons tend to be lost in p75−/−mice [46, 47] whereas other neuron populations (for example, sympathetic neurons) are increased in the absence of p75 [33].

The p75 receptor can promote neuronal apoptosis in the absence of Trk receptors [33, 34, 48]; for example, nerve growth factor causes the death of retinal neurons that express p75 but not TrkA [48]. Also, BDNF activates p75 to induce apoptosis of sympathetic neurons [33], which do not express TrkB. Based on these findings, we examined whether p75 causes the death of geniculate ganglion neurons in the absence of TrkB and whether the additional removal of p75 can rescue these neurons. Surprisingly, TrkB−/−/p75−/−mice had similar numbers of neurons as TrkB−/−mice. This finding suggests that p75 plays a different role in the development of the taste system compared with other systems [33, 48, 49], perhaps due to differences among systems in Trk receptor function. In the retina [48], sympathetic ganglia [33], and dorsal root ganglia [49], p75 induces apoptosis specifically in the absence of TrkA. Thus, TrkB may regulate cell death differently than TrkA [49], and the actions of p75 may differ in the absence of these two receptor types. Regardless of the underlying reason, our results indicate that p75 does not cause neuronal apoptosis when TrkB is absent during taste system development.

Because taste buds require support from nerve fibers to retain their normal numbers and size [50–53], taste buds are lost in the absence of neurotrophins [28–30, 54]. Likewise, since innervation to the tongue was disrupted, p75 mice also had a loss of taste buds. In all cases with neurotrophin knockout mice some un-innervated taste buds, as defined by keratin 8 labeling, remain [15, 54]. These taste bud remnants also remain following nerve section [50, 55]. Consistent with this literature we found un-innervated taste buds that remained in p75−/−mice. Because nerve section results in a greater loss of taste buds during development than it does in adulthood [53, 56, 57], we thought that perhaps these additional taste buds would be lost postnatally, but that was not the case; they still remained in P10 p75−/−mice. It seems likely that these smaller remaining un-innervated collections of K8-positive cells simply do not require innervation to be maintained.

Not all neurotrophin knockout and receptor mice lose the same number of taste buds; for example, TrkB−/−mice possess more taste buds than hybrid Bdnf−/−/Nt4−/−mice at birth [15, 17, 58]. The reason for this is unclear but we speculated that the additional taste buds might be supported by BDNF or NT4 functioning via p75. However, mice with both TrkB and p75 mutations possessed more taste buds than TrkB−/−mice, suggesting that p75 is not required for the additional taste buds in TrkB−/−mice. Although this increase in number of taste buds was surprising, p75 has been found to cause the death of neurons in the absence of Trk expression [33, 34, 48]. When we investigated whether the increase in taste bud number in TrkB−/−/p75−/−mice might be supported by a larger number of neurons, we found that geniculate ganglion neuron number was unaffected by mutation of both TrkB and p75, and few of the taste buds in TrkB−/−/p75−/−mice appeared to be innervated. Therefore, p75 does not cause the death of neurons in the absence of Trk signaling in the taste system, and the additional taste buds in TrkB−/−/p75−/−mice relative to TrkB−/−mice are not rescued by a greater number of neurons. Together, these findings suggest that, although p75 regulates innervation of the tongue surface, it does not mediate the role of BDNF in axon guidance or the role of TrkB ligands in neuron survival.

Conclusions

The pan-neurotrophic factor receptor, p75, regulates gustatory innervation patterns and branching of gustatory fibers in the tongue. Although gustatory neurons are lost in p75−/−mice this appears to be downstream of the innervation deficits, and is not likely due to a direct role of p75 in mediating BDNF- and/or NT4-regulated survival. The taste buds previously shown to be remaining in TrkB−/−mice, but not Bdnf−/−/Nt4−/−mice, are not maintained by p75 and leave open the possibility that the two factors function through an additional receptor other than TrkB and p75 to regulate gustatory development.

Methods

Animals

Heterozygous TrkB+/− (stock no. 002544; Jackson Laboratories, Bar Harbor, ME, USA) and p75+/− (stock no. 002213; Jackson Laboratories) mice were bred to produce p75−/−and TrkB−/−/p75−/−mice. The day that a sperm plug was positively identified was designated E0.5. Mice were genotyped using polymerase chain reaction. All procedures were approved by the University of Louisville Institutional Animal Care and Use Committee committee and are in accordance with the guidelines of the US Public Health Service Policy on Humane Care and Use of Laboratory Animals and the National Institutes of Health Guide for the Care and Use of Laboratory Animals.

Quantification of geniculate ganglion neurons

E13.5 (p75−/−n = 3, TrkB−/−/p75−/−n = 2, and wild-type n = 3), E14.5 (p75−/−n = 3, and wild-type n = 3), E16.5 (p75−/−n = 3, and wild-type n = 3), and E18.5 (p75−/−n = 3, and wild-type n = 3) embryos were transcardially perfused with ice-cold 4% phosphate-buffered paraformaldehyde (PFA) and post-fixed overnight in 4% PFA. Following fixation, embryo heads were dissected, transferred to 70% ethanol, and processed for paraffin embedding. Geniculate ganglion neurons were visualized using TUJ-1 antibody as previously described [5]. Briefly, serial sections (5 μm) of paraffin-embedded embryos were collected on SuperFrost Plus slides (Fisher Scientific, Waltham, MA, USA). Paraffin was then removed by immersion in Citrisolv (Fisher Scientific) overnight. Following rehydration and endogenous peroxidase blocking, slides were treated for antigen retrieval in citrate buffer (0.1 M citric acid and 0.1 M sodium citrate in dH2O; pH 6). Sections were washed in PBS, blocked for 1 hour in blocking solution (5% goat serum and 0.25% Triton X-100 in PBS), and incubated overnight in blocking solution containing mouse TUJ-1 antibody (1:500; #MMS-435P, Covance, Princeton, NJ, USA). The next day, sections were washed and incubated for 1.5 hours in blocking solution containing biotinylated anti-mouse secondary antibody (1:250; #BA-2000, Vector Laboratories, Burlingame, CA, USA) and visualized using an ABC diaminobenzidine reaction kit (#PK-6200; Vector Laboratories).

TUJ-1 immunostaining was used to quantify geniculate ganglion neurons in sections in which nuclei were visible. Neurons were counted in six sections per ganglion. The area of the geniculate ganglion in each of these six sections was measured, multiplied by section thickness, and summed to provide the total volume of quantified sections. The volume of the entire geniculate ganglion was measured in the same way, using every section containing the geniculate ganglion. The total number of neurons in the ganglion was estimated by multiplying the number of neurons per volume of the quantified sections by total ganglion volume. This number was multiplied by a correction factor to compensate for the presence of individual nuclei appearing in multiple sections [59]. This correction factor was calculated as N = n × [T/(T × D)], where N is the estimated total number of neurons, n is the number of neurons counted, T is the section thickness, and D is the average diameter of nuclei [5].

Quantification of taste buds

Mice at day of birth (p75−/−n = 5, TrkB−/−/p75−/−n = 3, and wild-type n = 4) and P10 (p75−/−n = 3 and wild-type n = 3) were anesthetized and transcardially perfused with ice-cold 4% PFA. The front of the tongue containing the fungiform field was separated and post-fixed in 4% PFA for 2 hours. Tongues were then placed in 30% sucrose overnight. The next day, tongues were embedded in OCT medium (#4583; Sakura Finetek USA, Inc., Torrance, CA, USA). Serial sagittal sections (25 μm) were collected onto SuperFrost Plus slides (Fisher Scientific). For antigen retrieval, sections were heat-dried overnight, rehydrated, placed into citrate buffer (pH 6.0), heated for 15 minutes in a boiling water bath, and incubated for 10 minutes at room temperature.

Primary rat anti-cytokeratin 8 antibody (1:50; Developmental Studies Hybridoma Bank, Iowa City, IA, USA) and rabbit anti-P2X3 antibody (#AB5895; 1:500, Millipore, Billerica, MA, USA) were used to label gustatory nerves and taste buds. Cytokeratin 8 is a marker of simple epithelia that labels most columnar taste cells [60, 61], and P2X3 is an ATP channel that is required for taste function [62] and expressed in most geniculate ganglion neurons innervating the tongue [63]. Secondary anti-rat Alexa 488 (green) and anti-rabbit Alexa 555 (red) antibodies (1:500, Molecular Probes, Grand Island, NY, USA) were used to visualize cytokeratin 8- and P2X3-containing nerve fibers, respectively. Sections were examined in order, and individual taste buds were followed across sections so that each taste bud was only counted once.

To assess innervation of taste buds, confocal stacks of optical sections with a Z-step of 0.5 μm were imaged for three to five taste buds from each mouse (p75−/−n = 5, and wild-type n = 4) and analyzed using ImageJ software (http://rsbweb.nih.gov/ij/). The area of the taste bud in each optical section was measured, and these areas were summed and multiplied by section thickness to calculate taste bud volume. The area of P2X3-positive staining within outlined taste buds was also measured in each optical section, and these areas were summed and multiplied by section thickness (0.5 μm) to calculate the volume of P2X3 labeling within taste buds. The percentage of taste bud volume occupied by innervation was calculated by dividing the volume of P2X3 labeling by the volume of cytokeratin 8 labeling.

DiI-labeling of the geniculate ganglia

E14.5 (p75−/−n = 3, wild-type n = 3), E16.5 (p75−/−n = 6, wild-type n = 5), and E18.5 (p75−/−n = 4, wild-type n = 6) mice were anesthetized and transcardially perfused in ice-cold 4% PFA. E13.5 mice were immersion-fixed. DiI labeling was performed as previously described [64]. Briefly, brains were removed and DiI crystals were placed on the geniculate ganglion and facial nerve. Embryos were incubated at 37°C for 2 to 8 weeks depending on their age. The tongue was then dissected, examined, and photographed using a fluorescent dissecting microscope (Leica Microsystems, Wetzlar, Germany) equipped with a camera (QImaging, 19535 56th Avenue, Suite 101 Surrey, BC ).

Data analysis

The total number of neurons was compared between genotypes on E13.5, E14.5, E16.5, and E18.5 using two-way analysis of variance. Geniculate ganglion neuron number and taste bud number and volume were compared using one-way analysis of variance. Statistical significance was set at P < 0.05. Data are reported as mean ± standard error of the mean in the text and figures.

Abbreviations

BDNF:

brain-derived neurotrophic factor

DiI:

(2Z)-2-[(E)-3-(3,3-dimethyl-1-octadecylindol-1-ium-2-yl)prop-2-enylidene]-3,3-dimethyl-1-octadecylindole perchlorate

E:

embryonic day

NT4:

neurotrophin-4

P:

postnatal day

P2X3:

purinoceptor 3

p75:

pan-neurotrophin receptor

PBS:

phosphate-buffered saline

PFA:

paraformaldehyde

Sema3A:

semaphorin 3A

TrkA:

tropomyosin related kinase A

TrkB:

tropomyosin related kinase B

TUJ1:

anti-β-III tubulin antibody.

References

  1. Mbiene JP, Mistretta CM: Initial innervation of embryonic rat tongue and developing taste papillae: nerves follow distinctive and spatially restricted pathways. Acta Anat (Basel). 1997, 160: 139-158.

    Article  CAS  Google Scholar 

  2. Lopez GF, Krimm RF: Refinement of innervation accuracy following initial targeting of peripheral gustatory fibers. J Neurobiol. 2006, 66: 1033-1043.

    Article  PubMed Central  PubMed  Google Scholar 

  3. Krimm RF, Hill DL: Innervation of single fungiform taste buds during development in rat. J Comp Neurol. 1998, 398: 13-24.

    Article  CAS  PubMed  Google Scholar 

  4. Conover JC, Erickson JT, Katz DM, Bianchi LM, Poueymirou WT, McClain J, Pan L, Helgren M, Ip NY, Boland P, Friedman B, Wiegand S, Vejsada R, Kato AC, Dechiara M, Yancopoulos GD: Neuronal deficits, not involving motor neurons, in mice lacking BDNF and/or NT4. Nature. 1995, 375: 235-238.

    Article  CAS  PubMed  Google Scholar 

  5. Patel AV, Krimm RF: BDNF is required for the survival of differentiated geniculate ganglion neurons. Dev Biol. 2010, 340: 419-429.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  6. Hoshino N, Vatterott P, Egwiekhor A, Rochlin MW: Brain-derived neurotrophic factor attracts geniculate ganglion neurites during embryonic targeting. Dev Neurosci. 2010, 32: 184-196.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  7. Lopez GF, Krimm RF: Epithelial overexpression of BDNF and NT4 produces distinct gustatory axon morphologies that disrupt initial targeting. Dev Biol. 2006, 292: 457-468.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  8. Ma L, Lopez GF, Krimm RF: Epithelial-derived brain-derived neurotrophic factor is required for gustatory neuron targeting during a critical developmental period. J Neurosci. 2009, 29: 3354-3364.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  9. Ringstedt T, Ibanez CF, Nosrat CA: Role of brain-derived neurotrophic factor in target invasion in the gustatory system. J Neurosci. 1999, 19: 3507-3518.

    CAS  PubMed  Google Scholar 

  10. Liu X, Ernfors P, Wu H, Jaenisch R: Sensory but not motor neuron deficits in mice lacking NT4 and BDNF. Nature. 1995, 375: 238-241.

    Article  CAS  PubMed  Google Scholar 

  11. Patel AV, Krimm RF: Neurotrophin-4 regulates the survival of gustatory neurons earlier in development using a different mechanism than brain-derived neurotrophic factor. Dev Biol. 2012, 365: 50-60.

    Article  CAS  PubMed  Google Scholar 

  12. Huang T, Krimm RF: Developmental expression of Bdnf, Ntf4/5, and TrkB in the mouse peripheral taste system. Dev Dyn. 2010, 239: 2637-2646.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  13. Huang T, Krimm RF: BDNF and NT4 play interchangeable roles in gustatory development. Dev Biol. 2014, 386: 308-320.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  14. Berkemeier LR, Winslow JW, Kaplan DR, Nikolics K, Goeddel DV, Rosenthal A: Neurotrophin-5: a novel neurotrophic factor that activates trk and trkB. Neuron. 1991, 7: 857-866.

    Article  CAS  PubMed  Google Scholar 

  15. Fritzsch B, Sarai PA, Barbacid M, Silos-Santiago I: Mice with a targeted disruption of the neurotrophin receptor trkB lose their gustatory ganglion cells early but do develop taste buds. Int J Dev Neurosci. 1997, 15: 563-576.

    Article  CAS  PubMed  Google Scholar 

  16. Klein R, Nanduri V, Jing SA, Lamballe F, Tapley P, Bryant S, Cordon-Cardo C, Jones KR, Reichardt LF, Barbacid M: The trkB tyrosine protein kinase is a receptor for brain-derived neurotrophic factor and neurotrophin-3. Cell. 1991, 66: 395-403.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  17. Fei D, Krimm RF: Taste neurons consist of both a large TrkB-receptor-dependent and a small TrkB-receptor-independent subpopulation. PLoS One. 2013, 8: e83460-

    Article  PubMed Central  PubMed  Google Scholar 

  18. Krimm RF: Mice lacking the p75 receptor fail to acquire a normal complement of taste buds and geniculate ganglion neurons by adulthood. Anat Rec A Discov Mol Cell Evol Biol. 2006, 288: 1294-1302.

    Article  PubMed Central  PubMed  Google Scholar 

  19. Blochl A, Blochl R: A cell-biological model of p75NTR signaling. J Neurochem. 2007, 102: 289-305.

    Article  CAS  PubMed  Google Scholar 

  20. Chao MV: The p75 neurotrophin receptor. J Neurobiol. 1994, 25: 1373-1385.

    Article  CAS  PubMed  Google Scholar 

  21. DeFreitas MF, McQuillen PS, Shatz CJ: A novel p75NTR signaling pathway promotes survival, not death, of immunopurified neocortical subplate neurons. J Neurosci. 2001, 21: 5121-5129.

    CAS  PubMed  Google Scholar 

  22. Hempstead BL: The many faces of p75NTR. Curr Opin Neurobiol. 2002, 12: 260-267.

    Article  CAS  PubMed  Google Scholar 

  23. Lopez-Sanchez N, Frade JM: Control of the cell cycle by neurotrophins: lessons from the p75 neurotrophin receptor. Histol Histopathol. 2002, 17: 1227-1237.

    CAS  PubMed  Google Scholar 

  24. Roux PP, Barker PA: Neurotrophin signaling through the p75 neurotrophin receptor. Prog Neurobiol. 2002, 67: 203-233.

    Article  CAS  PubMed  Google Scholar 

  25. Teng HK, Teng KK, Lee R, Wright S, Tevar S, Almeida RD, Kermani P, Torkin R, Chen ZY, Lee FS, Kraemer RT, Nykjaer A, Hempstead BL: ProBDNF induces neuronal apoptosis via activation of a receptor complex of p75NTR and sortilin. J Neurosci. 2005, 25: 5455-5463.

    Article  CAS  PubMed  Google Scholar 

  26. Yamashita T, Fujitani M, Hata K, Mimura F, Yamagishi S: Diverse functions of the p75 neurotrophin receptor. Anat Sci Int. 2005, 80: 37-41.

    Article  CAS  PubMed  Google Scholar 

  27. Mistretta CM, Hill DL: Development of the taste system: basic neurobiology. Handbook of Olfaction and Gustation. Edited by: RichardL D. 2003, New York: Marcel Dekker, 759-782.

    Google Scholar 

  28. Mistretta CM, Goosens KA, Farinas I, Reichardt LF: Alterations in size, number, and morphology of gustatory papillae and taste buds in BDNF null mutant mice demonstrate neural dependence of developing taste organs. J Comp Neurol. 1999, 409: 13-24.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  29. Nosrat CA, Blomlof J, ElShamy WM, Ernfors P, Olson L: Lingual deficits in BDNF and NT3 mutant mice leading to gustatory and somatosensory disturbances, respectively. Development. 1997, 124: 1333-1342.

    CAS  PubMed  Google Scholar 

  30. Sun H, Oakley B: Development of anterior gustatory epithelia in the palate and tongue requires epidermal growth factor receptor. Dev Biol. 2002, 242: 31-43.

    Article  CAS  PubMed  Google Scholar 

  31. Hosley MA, Hughes SE, Morton LL, Oakley B: A sensitive period for the neural induction of taste buds. J Neurosci. 1987, 7: 2075-2080.

    CAS  PubMed  Google Scholar 

  32. Whitehead MC, Kachele DL: Development of fungiform papillae, taste buds, and their innervation in the hamster. J Comp Neurol. 1994, 340: 515-530.

    Article  CAS  PubMed  Google Scholar 

  33. Bamji SX, Majdan M, Pozniak CD, Belliveau DJ, Aloyz R, Kohn J, Causing CG, Miller FD: The p75 neurotrophin receptor mediates neuronal apoptosis and is essential for naturally occurring sympathetic neuron death. J Cell Biol. 1998, 140: 911-923.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  34. Deppmann CD, Mihalas S, Sharma N, Lonze BE, Niebur E, Ginty DD: A model for neuronal competition during development. Science. 2008, 320: 369-373.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  35. Farinas I, Wilkinson GA, Backus C, Reichardt LF, Patapoutian A: Characterization of neurotrophin and Trk receptor functions in developing sensory ganglia: direct NT-3 activation of TrkB neurons in vivo. Neuron. 1998, 21: 325-334.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  36. Rochlin MW, Farbman AI: Trigeminal ganglion axons are repelled by their presumptive targets. J Neurosci. 1998, 18: 6840-6852.

    CAS  PubMed  Google Scholar 

  37. Rochlin MW, O'Connor R, Giger RJ, Verhaagen J, Farbman AI: Comparison of neurotrophin and repellent sensitivities of early embryonic geniculate and trigeminal axons. J Comp Neurol. 2000, 422: 579-593.

    Article  CAS  PubMed  Google Scholar 

  38. Runge EM, Hoshino N, Biehl MJ, Ton S, Rochlin MW: Neurotrophin-4 is more potent than brain-derived neurotrophic factor in promoting, attracting and suppressing geniculate ganglion neurite outgrowth. Dev Neurosci. 2012, 34 (5): 389-401.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  39. Bentley CA, Lee KF: p75 is important for axon growth and Schwann cell migration during development. J Neurosci. 2000, 20: 7706-7715.

    CAS  PubMed  Google Scholar 

  40. Lim YS, McLaughlin T, Sung TC, Santiago A, Lee KF, O'Leary DD: p75(NTR) mediates ephrin-A reverse signaling required for axon repulsion and mapping. Neuron. 2008, 59: 746-758.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  41. Ben-Zvi A, Ben-Gigi L, Klein H, Behar O: Modulation of semaphorin3A activity by p75 neurotrophin receptor influences peripheral axon patterning. J Neurosci. 2007, 27: 13000-13011.

    Article  CAS  PubMed  Google Scholar 

  42. Vilbig R, Cosmano J, Giger R, Rochlin MW: Distinct roles for Sema3A, Sema3F, and an unidentified trophic factor in controlling the advance of geniculate axons to gustatory lingual epithelium. J Neurocytol. 2004, 33: 591-606.

    Article  PubMed  Google Scholar 

  43. Dillon TE, Saldanha J, Giger R, Verhaagen J, Rochlin MW: Sema3A regulates the timing of target contact by cranial sensory axons. J Comp Neurol. 2004, 470: 13-24.

    Article  CAS  PubMed  Google Scholar 

  44. Perrelet D, Ferri A, Liston P, Muzzin P, Korneluk RG, Kato AC: IAPs are essential for GDNF-mediated neuroprotective effects in injured motor neurons in vivo. Nat Cell Biol. 2002, 4: 175-179.

    Article  CAS  PubMed  Google Scholar 

  45. Lee KF, Li E, Huber LJ, Landis SC, Sharpe AH, Chao MV, Jaenisch R: Targeted mutation of the gene encoding the low affinity NGF receptor p75 leads to deficits in the peripheral sensory nervous system. Cell. 1992, 69: 737-749.

    Article  CAS  PubMed  Google Scholar 

  46. Sorensen B, Tandrup T, Koltzenburg M, Jakobsen J: No further loss of dorsal root ganglion cells after axotomy in p75 neurotrophin receptor knockout mice. J Comp Neurol. 2003, 459: 242-250.

    Article  CAS  PubMed  Google Scholar 

  47. Stucky CL, Koltzenburg M: The low-affinity neurotrophin receptor p75 regulates the function but not the selective survival of specific subpopulations of sensory neurons. J Neurosci. 1997, 17: 4398-4405.

    CAS  PubMed  Google Scholar 

  48. Frade JM, Rodriguez-Tebar A, Barde YA: Induction of cell death by endogenous nerve growth factor through its p75 receptor. Nature. 1996, 383: 166-168.

    Article  CAS  PubMed  Google Scholar 

  49. Nikoletopoulou V, Lickert H, Frade JM, Rencurel C, Giallonardo P, Zhang L, Bibel M, Barde YA: Neurotrophin receptors TrkA and TrkC cause neuronal death whereas TrkB does not. Nature. 2010, 467: 59-63.

    Article  CAS  PubMed  Google Scholar 

  50. Guagliardo NA, Hill DL: Fungiform taste bud degeneration in C57BL/6 J mice following chorda-lingual nerve transection. J Comp Neurol. 2007, 504: 206-216.

    Article  PubMed Central  PubMed  Google Scholar 

  51. Guth L: The effects of glossopharyngeal nerve transection on the circumvallate papilla of the rat. Anat Rec. 1957, 128: 715-731.

    Article  CAS  PubMed  Google Scholar 

  52. Naga IA, Sakla FB, Girgis ZA, State FA: Denervation of taste buds in the rabbit. Am J Anat. 1970, 129: 53-63.

    Article  CAS  PubMed  Google Scholar 

  53. Nagato T, Matsumoto K, Tanioka H, Kodama J, Toh H: Effect of denervation on morphogenesis of the rat fungiform papilla. Acta Anat (Basel). 1995, 153: 301-309.

    Article  CAS  Google Scholar 

  54. Patel AV, Huang T, Krimm RF: Lingual and palatal gustatory afferents each depend on both BDNF and NT-4, but the dependence is greater for lingual than palatal afferents. J Comp Neurol. 2010, 518: 3290-3301.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  55. Whitehead MC, Frank ME, Hettinger TP, Hou LT, Nah HD: Persistence of taste buds in denervated fungiform papillae. Brain research. 1987, 405: 192-195.

    Article  CAS  PubMed  Google Scholar 

  56. Hosley MA, Oakley B: Postnatal development of the vallate papilla and taste buds in rats. Anat Rec. 1987, 218: 216-222.

    Article  CAS  PubMed  Google Scholar 

  57. Sollars SI, Smith PC, Hill DL: Time course of morphological alterations of fungiform papillae and taste buds following chorda tympani transection in neonatal rats. J Neurobiol. 2002, 51: 223-236.

    Article  PubMed  Google Scholar 

  58. Ito A, Nosrat CA: Gustatory papillae and taste bud development and maintenance in the absence of TrkB ligands BDNF and NT-4. Cell Tissue Res. 2009, 337: 349-359.

    Article  CAS  PubMed  Google Scholar 

  59. Abercrombie M: Estimation of nuclear population from microtome sections. Anat Rec. 1946, 94: 239-247.

    Article  CAS  PubMed  Google Scholar 

  60. Knapp L, Lawton A, Oakley B, Wong L, Zhang C: Keratins as markers of differentiated taste cells of the rat. Differentiation. 1995, 58: 341-349.

    Article  CAS  PubMed  Google Scholar 

  61. Liebl DJ, Mbiene JP, Parada LF: NT4/5 mutant mice have deficiency in gustatory papillae and taste bud formation. Dev Biol. 1999, 213: 378-389.

    Article  CAS  PubMed  Google Scholar 

  62. Finger TE, Danilova V, Barrows J, Bartel DL, Vigers AJ, Stone L, Hellekant G, Kinnamon SC: ATP signaling is crucial for communication from taste buds to gustatory nerves. Science. 2005, 310: 1495-1499.

    Article  CAS  PubMed  Google Scholar 

  63. Ishida Y, Ugawa S, Ueda T, Yamada T, Shibata Y, Hondoh A, Inoue K, Yu Y, Shimada S: P2X(2)- and P2X(3)-positive fibers in fungiform papillae originate from the chorda tympani but not the trigeminal nerve in rats and mice. J Comp Neurol. 2009, 514: 131-144.

    Article  CAS  PubMed  Google Scholar 

  64. Krimm RF, Miller KK, Kitzman PH, Davis BM, Albers KM: Epithelial overexpression of BDNF or NT4 disrupts targeting of taste neurons that innervate the anterior tongue. Dev Biol. 2001, 232: 508-521.

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

We would like to thank Ms Darlene Burke for statistical support and Mr Bradley Biggs for breeding and genotyping many of the animals used in the study. This project was supported by NIDCD grant DC009418 to RFK, which funded the entire project except the statistical analysis. The statistical core facility is supported by NIH grant 8P30GM103507. Neither funding body participated in the design, in the collection, analysis, and interpretation of data, in the writing of the manuscript, or in the decision to submit the manuscript for publication.

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Authors and Affiliations

  1. Department of Anatomical Sciences and Neurobiology, University of Louisville School of Medicine, Louisville, KY, 40292, USA

    Da Fei, Tao Huang & Robin F Krimm

Authors
  1. Da Fei
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  2. Tao Huang
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  3. Robin F Krimm
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Correspondence to Robin F Krimm.

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Competing interests

The authors declare that they have no competing interests.

Authors’ contributions

DF carried out most of the studies and drafted the initial manuscript. TH carried out the E13.5 Di-labeling study. RFK conceived of the study, participated in its design, constructed most of the final figures, determined the percentage of taste buds that were innervated in p75−/−mice and drafted the final manuscript. All authors read and approved the final manuscript.

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Fei, D., Huang, T. & Krimm, R.F. The neurotrophin receptor p75 regulates gustatory axon branching and promotes innervation of the tongue during development. Neural Dev 9, 15 (2014). https://doi.org/10.1186/1749-8104-9-15

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Keywords

Neural Development

ISSN: 1749-8104